1. Biological applications of synthetic binders isolated from a conceptually new adhiron libraryClaudia D'Ercole, Matteo De March, Gianluca Veggiani, Sandra Oloketuyi, Rossella Svigelj, Ario De Marco, 2023, original scientific article Keywords: synthetic library, adhirons, affimers, C-reactive protein, SpyCatcher
Published in RUNG: 17.10.2023; Views: 2461; Downloads: 5
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3. A framework for evaluating the performance of SMLM cluster analysis algorithmsDaniel J. Nieves, Jeremy A. Pike, Florian Levet, David J. Williamson, Mohammed Baragilly, Sandra Oloketuyi, Ario De Marco, Juliette Griffié, Daniel Sage, Edward A. K. Cohen, 2023, original scientific article Keywords: super resolution microscopy, functionalized nanobodies
Published in RUNG: 22.02.2023; Views: 2580; Downloads: 0
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4. Native llama nanobody library panning performed by phage and yeast display provides binders suitable for C-reactive protein detectionSandra Oloketuyi, Robert Alvin Bernedo-Navarro, Andreas Christmann, Justyna Borkowska, Giulia Cazzaniga, Horst Wilhelm Schuchmann, Joanna Niedziółka-Jönsson, Katarzyna Szot-Karpińska, Harald Kolmar, Ario De Marco, 2021, original scientific article Keywords: phage display, yeast display, nanobodies, electrochemical impedance biosensors
Published in RUNG: 06.12.2021; Views: 3153; Downloads: 46
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5. CDR1 composition can affect nanobody recombinant expression yieldsMarco Orlando, Sara Fortuna, Sandra Oloketuyi, Gregor Bajc, Adi Goldenzweig, Ario De Marco, 2021, original scientific article Keywords: nanobodies, in silico engineering, mutagenesis
Published in RUNG: 15.09.2021; Views: 2937; Downloads: 46
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6. Nanobody-dependent detection of microcystis aeruginosa by ELISA and thermal lens spectrometryGbenga Folorunsho Oginni, Sandra Oloketuyi, Elisa Mazzega, Hanna Budasheva, Alfred Beran, Marina Cabrini, Dorota Korte, Mladen Franko, Ario De Marco, 2021, original scientific article Keywords: cyanobacteria, nanobodies, fluobodies, phage display, thermal lens spectrometry
Published in RUNG: 19.04.2021; Views: 3723; Downloads: 0
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7. An anti-HER2 nanobody binds to its antigen HER2 via two independent paratopesDaniele Ubbiali, Marco Orlando, Matic Kovačič, Claudio Iacobucci, Marta S. Semrau, Gregor Bajc, Sara Fortuna, Gregor Ilc, Barbara Medagli, Sandra Oloketuyi, Ario De Marco, 2021, original scientific article Keywords: NMR, cross-linking, mass spectrometry, nanobodies, modelling, rational mutagenesis
Published in RUNG: 15.04.2021; Views: 3820; Downloads: 0
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8. Effect of Humanizing Mutations on the Stability of the Llama Single-Domain Variable RegionMiguel Soler, Barbara Medagli, Jiewen Wang, Sandra Oloketuyi, Gregor Bajc, He Huang, Sara Fortuna, Ario De Marco, 2021, original scientific article Keywords: nanobody framework, modeling, nanobody humanization, CDR grafting
Published in RUNG: 27.01.2021; Views: 3717; Downloads: 68
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9. Diversity of bacteria and bacterial products as antibiofilm and antiquorum sensing drugs against pathogenic bacteriaFazlurrahman Khan, Sandra Oloketuyi, Young-Mog Kim, 2019, review article Abstract: The increase in antibiotic resistance of pathogenic bacteria has led to the development of new therapeutic approaches to inhibit biofilm formation as well as interfere quorum sensing (QS) signaling systems. The QS system is a phenomenon in which pathogenic bacteria produce signaling molecules that are involved in cell to cell communication, production of virulence factors, biofilm maturation, and several other functions. In the natural environment, several non-pathogenic bacteria are present as mixed population along with pathogenic bacteria and they control the behavior of microbial community by producing secondary metabolites. Similarly, non-pathogenic bacteria also take advantages of the QS signaling molecule as a sole carbon source for their growth through catabolism with enzymes. Several enzymes are produced by bacteria which disrupt the biofilm architecture by degrading the composition of extracellular polymeric substances (EPS) such as exopolysaccharide, extracellular- DNA and protein. Thus, the interference of QS system by bacterial metabolic products and enzymatic catalysis, modification of the QS signaling molecules as well as enzymatic disruption of biofilm architecture have been considered as the alternative therapeutic approaches. This review article elaborates on the diversity of different bacterial species with respect to their metabolic products as well as enzymes and their molecular modes of action. The bacterial enzymes and metabolic products will open new and promising perspectives for the development of strategies against the pathogenic bacterial infections.
Keywords: Bacteria, biofilm, quorum sensing, inhibition, metabolites, pathogen, virulence.
Published in RUNG: 18.01.2021; Views: 4496; Downloads: 0
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10. Treatment strategies targeting persister cell formation in bacterial pathogensFazlurrahman Khan, Dung Pham, Nazia Tabassum, Sandra Oloketuyi, Young-Mog Kim, 2020, review article Abstract: Persister cells are transiently antibiotic-tolerant and dormant subpopulations that are produced to escape the effects of antibiotics within biofilms or planktonic cell populations. Persister cells are of high clinical importance due to their tolerance to antimicrobial agents and subsequent failure in antibiotic treatments. Understanding persister cell formation mechanisms is therefore highly important for developing effective therapeutic strategies against pathogenic bacterial persisters. Several anti-persister compounds have been previously identified via isolation from natural resources or chemical synthesis. Furthermore, a combination of these compounds with antibiotics or non-antibiotic drugs also allows action on multiple targets while reducing the administration frequency. Here, we present a comprehensive overview of the clinical importance and formation mechanisms of persister cells as well as the current treatment strategies against persister cell formations in chronic infections.
Keywords: Biofilm, anti-persister agents, persister cells, pathogenic bacteria, chronic infections
Published in RUNG: 14.01.2021; Views: 4763; Downloads: 0
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