1. A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignanciesRoberto De Luca, Paul Kachel, Klara Kropivšek, Berend Snijder, Markus G. Manz, Dario Neri, 2018, original scientific article Abstract: A novel dual-cytokine–antibody fusion protein, consisting of an antibody directed against CD38 [a tumor-associated antigen mainly expressed on the surface of multiple myeloma (MM) cells], simultaneously fused to both tumor necrosis factor ligand superfamily member 10 (TRAIL) and interleukin-2 (IL2), was designed, expressed and purified to homogeneity. The novel fusion protein, termed IL2-αCD38-αCD38-scTRAIL, was able to selectively recognize its cognate antigen expressed on the surface of MM and lymphoma cell lines, as evidenced by flow cytometry analysis. Moreover, the targeted version of TRAIL was able to induce cancer cell death in vitro, both with MM cell lines and with fresh isolates from the bone marrow of MM patients. The experiments provide a rationale for possible future applications of IL2-αCD38-αCD38-scTRAIL for the treatment of patients with MM or other CD38-positive malignancies.
Keywords: antibodies, dual-cytokine-antibody, CD38, multiple myeloma, TRAIL, IL2
Published in RUNG: 11.11.2024; Views: 584; Downloads: 2
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3. Diagnostics and Monitoring of COVID-19 Infection – Current UnderstandingMartina Bergant Marušič, Ario De Marco, 2020, review, book review, critique Keywords: SARS-CoV-2, RT-PCR, seroconversion, serum biomarkers, neutralizing antibodies
Published in RUNG: 30.09.2022; Views: 2493; Downloads: 24
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5. Comparative analysis of fusion tags used to functionalize recombinant antibodiesArio De Marco, Gianluca Veggiani, Barbara Giabbai, Marta Semrau, Barbara Medagli, Vincenzo Riccio, Gregor Bajc, Paola Storici, 2020, original scientific article Keywords: recombinant antibodies, protein production, fusion tags
Published in RUNG: 30.09.2019; Views: 4092; Downloads: 71
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9. Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodiesArio De Marco, Selma Djender, Anne Beugnet, Aurelie Schneider, Ronan Crepin, Klervi Even Desrumeaux, Chiara Romani, Sandrine Moutel, Franck Perez, 2014, original scientific article Keywords: recombinant antibodies, cytoplasmic expression, functional tags
Published in RUNG: 16.06.2016; Views: 5467; Downloads: 135
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