Repository of University of Nova Gorica

Search the repository
A+ | A- | Help | SLO | ENG

Query: search in
search in
search in
search in
* old and bolonia study programme

Options:
  Reset


1 - 2 / 2
First pagePrevious page1Next pageLast page
1.
Temporal and spatial patterns of zinc and iron accumulation during barley (Hordeum vulgare L.) grain development. Journal of agricultural and food chemistry.
Amelie Detterbeck, Paula Pongrac, Daniel Persson, Iztok Arčon, Primož Pelicon, Primož Vaupetič, Mitja Kelemen, Søren Husted, Katarina Vogel-Mikuš, Stephan Clemens, Jan Kofod Shjoerring, 2020, original scientific article

Abstract: Breeding and engineering of biofortified crops will benefit from a better understanding of bottlenecks controlling micronutrient loading within the seeds. However, few studies have addressed the changes in micronutrient concentrations, localization, and speciation occurring over time. Therefore, we studied spatial patterns of zinc and iron accumulation during grain development in two barley lines with contrasting grain zinc concentrations. Microparticle-induced-X-ray emission and laser ablationinductively coupled plasma mass spectrometry were used to determine tissue-specific accumulation of zinc, iron, phosphorus, and sulfur. Differences in zinc accumulation between the lines were most evident in the endosperm and aleurone. A gradual decrease in zinc concentrations from the aleurone to the underlying endosperm was observed, while iron and phosphorus concentrations decreased sharply. Iron co-localized with phosphorus in the aleurone, whereas zinc co-localized with sulfur in the sub-aleurone. We hypothesize that differences in grain zinc are largely explained by the endosperm storage capacity. Engineering attempts should be targeted accordingly.
Found in: ključnih besedah
Summary of found: ...barley (Hordeum vulgare L.), biofortification, grain development, grain loading, LA-ICP-MS, μ-PIXE...
Keywords: barley (Hordeum vulgare L.), biofortification, grain development, grain loading, LA-ICP-MS, μ-PIXE
Published: 20.10.2020; Views: 891; Downloads: 0
.pdf Fulltext (4,76 MB)

2.
Mineral element composition in grain of awned and awnletted wheat (Triticum aestivum L.) cultivars tissue-specific iron speciation and phytate and non-phytate ligand ratio
Hiram Castillo Michel, Iztok Arčon, Paula Pongrac, Katarina Vogel-Mikuš, 2020, original scientific article

Abstract: In wheat (Triticum aestivum L.), the awns—the bristle-like structures extending from lemmas—are photosynthetically active. Compared to awned cultivars, awnletted cultivars produce more grains per unit area and per spike, resulting in significant reduction in grain size, but their mineral element composition remains unstudied. Nine awned and 11 awnletted cultivars were grown simultaneously in the field. With no difference in 1000-grain weight, a larger calcium and manganese—but smaller iron (Fe) concentrations—were found in whole grain of awned than in awnletted cultivars. Micro X-ray absorption near edge structure analysis of different tissues of frozen-hydrated grain cross-sections revealed that differences in total Fe concentration were not accompanied by differences in Fe speciation (64% of Fe existed as ferric and 36% as ferrous species) or Fe ligands (53% were phytate and 47% were non-phytate ligands). In contrast, there was a distinct tissue-specificity with pericarp containing the largest proportion (86%) of ferric species and nucellar projection (49%) the smallest. Phytate ligand was predominant in aleurone, scutellum and embryo (72%, 70%, and 56%, respectively), while nucellar projection and pericarp contained only non-phytate ligands. Assuming Fe bioavailability depends on Fe ligands, we conclude that Fe bioavailability from wheat grain is tissue specific.
Found in: ključnih besedah
Summary of found: ... biofortification, phytate, iron, awn, X-ray fluorescence, X-ray absorption...
Keywords: biofortification, phytate, iron, awn, X-ray fluorescence, X-ray absorption spectrometry, phosphorus, sulphur, nicotianamine
Published: 16.01.2020; Views: 1175; Downloads: 0
.pdf Fulltext (2,63 MB)

Search done in 0 sec.
Back to top