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1.
Fluorescent covalent organic frameworks : promising bioimaging materials
Chimatahalli Santhakumar Karthik, Tina Škorjanc, Dinesh Shetty, 2024, original scientific article

Abstract: Fluorescent covalent organic frameworks (COFs) have emerged as promising candidates for imaging living cells due to their unique properties and adjustable fluorescence. In this mini-review, we provide an overview of recent advancements in fluorescent COFs for bioimaging applications. We discuss the strategies used to design COFs with desirable properties such as high photostability, excellent biocompatibility, and pH sensitivity. Additionally, we explore the various ways in which fluorescent COFs are utilized in bioimaging, including cellular imaging, targeting specific organelles, and tracking biomolecules. We delve into their applications in sensing intracellular pH, reactive oxygen species (ROS), and specific biomarkers. Furthermore, we examine how functionalization techniques enhance the targeting and imaging capabilities of fluorescent COFs. Finally, we discuss the challenges and prospects in the field of fluorescent COFs for bioimaging in living cells, urging further research in this exciting area.
Keywords: covalent organic frameworks, fluorescent materials, imaging, bioimaging, biosensors
Published in RUNG: 05.03.2024; Views: 2312; Downloads: 8
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2.
Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omics
Dino Metarapi, Johannes Teun van Elteren, Martin Šala, Katarina Vogel-Mikuš, Iztok Arčon, Vid Simon Šelih, Mitja Kolar, Samo B. Hočevar, 2021, original scientific article

Abstract: Advanced data processing and visualization techniques were applied to laser ablation-single particleinductively coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization strategies are shown with special emphasis on spatially resolved information associated with the number and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained by combining the results of techniques from several complementary platforms.
Keywords: Laser ablation, Ag XANES, bioimaging
Published in RUNG: 03.06.2021; Views: 3606; Downloads: 52
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