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Laser ablation-single-particle-inductively coupled plasma mass spectrometry as a multimodality bioimaging tool in nano-based omicsSamo B. Hočevar,
Mitja Kolar,
Vid Simon Šelih,
Iztok Arčon,
Katarina Vogel-Mikuš,
Martin Šala,
Johannes Teun van Elteren,
Dino Metarapi, 2021, original scientific article
Abstract: Advanced data processing and visualization techniques were applied to laser ablation-single particleinductively
coupled plasma mass spectrometry (LA-sp-ICPMS) data obtained from imaging of roots of
sunflower (Helianthus annuus L.) which were exposed to ionic silver (Ag+). Highly multiplexed images with
tissue level resolution were generated, showing the uptake and transformation of Ag+ to silver
nanoparticles (AgNPs) in whole root cross-sections, and revealing that the size of biosynthesized AgNPs is
predisposed by the reducing power of particular root compartments. Various Ag+ and AgNP visualization
strategies are shown with special emphasis on spatially resolved information associated with the number
and spherical-equivalent size of individual AgNPs in user-selected root cross-sectional regions, including
statistical analysis of the AgNP distribution. Normally, this multimodality information can only be obtained
by combining the results of techniques from several complementary platforms.
Found in: ključnih besedah
Keywords: Laser ablation, Ag XANES, bioimaging
Published: 03.06.2021; Views: 1254; Downloads: 43
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