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Abstract: Capacitive field-effect biosensors modified with a stacked polyelectrolyte/enzyme urease bilayer were applied for the detection of the urinary biomarker urea in phosphate buffer and artificial urine. A theoretical kinetic model of the biosensor was developed and compared with the experimentally observed results.
Keywords: field-effect sensors, urea biosensors, polyelectrolyte, urease, artificial urine
Published in RUNG: 02.10.2024; Views: 122; Downloads: 1
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Keywords: biosensors, chronic diseases, biomarkers
Published in RUNG: 14.08.2024; Views: 587; Downloads: 0
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Abstract: Fluorescent covalent organic frameworks (COFs) have emerged as promising candidates for imaging living cells due to their unique properties and adjustable fluorescence. In this mini-review, we provide an overview of recent advancements in fluorescent COFs for bioimaging applications. We discuss the strategies used to design COFs with desirable properties such as high photostability, excellent biocompatibility, and pH sensitivity. Additionally, we explore the various ways in which fluorescent COFs are utilized in bioimaging, including cellular imaging, targeting specific organelles, and tracking biomolecules. We delve into their applications in sensing intracellular pH, reactive oxygen species (ROS), and specific biomarkers. Furthermore, we examine how functionalization techniques enhance the targeting and imaging capabilities of fluorescent COFs. Finally, we discuss the challenges and prospects in the field of fluorescent COFs for bioimaging in living cells, urging further research in this exciting area.
Keywords: covalent organic frameworks, fluorescent materials, imaging, bioimaging, biosensors
Published in RUNG: 05.03.2024; Views: 1346; Downloads: 6
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Abstract: Porous organic polymers (POPs) and covalent organic frameworks (COFs) have gained significant attention in the scientific community for a wide array of applications because of their attractive physical and chemical properties. Porosity of these materials provides ample surface area for interaction with targets, while crystallinity allows for highly specific structural tuning. In this seminar, I will present two strategies of utilizing these features of newly prepared materials in biosensing. Firstly, a cationic POP was synthesized, deposited onto interdigitated electrode arrays via a nontraditional electrophoresis technique, and utilized for electrochemical sensing of bacterial cells. As the principle of detection relied on electrostatic interactions between the cationic POP and the anionic bacterial surface, the sensor operated for both Gram-positive and Gram-negative bacteria. Secondly, a small-molecule nitroimidazole target for hypoxia, a low oxygen environment present in tumors, was post-synthetically conjugated to the pores of a fluorescent COF. This material served as a useful hypoxia imaging agent in cancerous cells. The seminar will conclude with some future perspectives on POPs and COFs in biological applications followed by Q & A.
Keywords: Porous organic polymers, covalent organic frameworks, biosensors, hypoxia
Published in RUNG: 13.07.2023; Views: 1482; Downloads: 0
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Keywords: phage display, yeast display, nanobodies, electrochemical impedance biosensors
Published in RUNG: 06.12.2021; Views: 2296; Downloads: 42
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