1. Exploring innovative approaches in flavored ciders : physicochemical and antioxidative properties of cornelian cherry – apple ciderErika Jež, Marianna Mazur, Tatjana Radovanović Vukajlović, Mitja Martelanc, Bartłomiej Menażyk, Maja Pečarič, Jelena Topić, Melita Sternad Lemut, Guillaume Antalick, Branka Mozetič Vodopivec, Lorena Butinar, 2024, published scientific conference contribution abstract Keywords: Cornelian cherry, apple cider, antioxidative properties Published in RUNG: 27.11.2024; Views: 224; Downloads: 0 Link to file This document has many files! More... |
2. Chemical characterization of cider produced in hardanger : from juice to finished ciderIngunn Ovsthus, Mitja Martelanc, Alen Albreht, Tatjana Radovanović Vukajlović, Urban Česnik, Branka Mozetič Vodopivec, 2024, original scientific article Abstract: Our investigation delves into the previously uncharted territory of cider composition from Norway. This study aimed to obtain an overview of the qualitative and quantitative compositions of general chemical parameters, polyphenols (individual and total expressed as gallic acids equivalents), selected esters, and selected C6-alcohols in ciders with the PDO label Cider from Hardanger. In total, 45 juice and cider samples from the fermentation process were collected from 10 cider producers in Hardanger in 2019, 2020, and 2021. Individual sugars, acids, ethanol, and 13 individual phenols were quantified using HPLC-UV/RI. Seven ethyl esters of fatty acids, four ethyl esters of branched fatty acids, ten acetate esters, two ethyl esters of hydroxycinnamic acids, and four C6-alcohols were quantified using HS-SPME-GC-MS. For samples of single cultivars (‘Aroma’, ‘Discovery’, ‘Gravenstein’, and ‘Summerred’), the sum of the measured individual polyphenols in the samples ranges, on average, from 79 to 289 mg L−1 (the lowest for ‘Summerred’ and highest for ‘Discovery’ and ‘Gravenstein’). Chlorogenic acid was the most abundant polyphenol in all samples. Ethyl butyrate, ethyl hexanoate, ethyl octanoate, ethyl decanoate, ethyl isobutyrate, ethyl 2-methylbutyrate, isoamyl acetate, and hexanol were present at concentrations above the odour threshold and contributed to the fruity flavour of the Cider from Hardanger. Keywords: cider, volatile aroma, esters, higher alcohols, polyphenols Published in RUNG: 14.08.2024; Views: 864; Downloads: 6 Full text (1,02 MB) This document has many files! More... |
3. Osnove senzorike : predavanje na usposabljanju Izobraževanje o osnovah senzorike, mikrovinifikacije in kemijskih lastnosti vin, Vipava, Center za raziskave vina, 10. maj 2024Marko Lesica, 2024, other performed works Abstract: Strokovno usposabljanje partnerjev pilotnega projekta EIP DRES - Testiranje uporabe drenovih jagod pri proizvodnji sadnih vin in partnerjev pilotnega projekta EIP PA-HAS - Pilotna alkoholna pijača z uporabo haskap jagod. Usposabljanje je s področja osnov senzorične analize alkoholnih pijač Keywords: cider, senzorična analiza, poskusne fermentacije, drenove jagode, haskap jagode Published in RUNG: 09.08.2024; Views: 806; Downloads: 0 This document has many files! More... |
4. Chemical composition of apple cider : a comparative study of Norwegian and French cidersIngunn Ovsthus, Mitja Martelanc, Tatjana Radovanović Vukajlović, Marko Lesica, Lorena Butinar, Branka Mozetič Vodopivec, Guillaume Antalick, 2024, published scientific conference contribution Abstract: Norwegian apple ciders have recently gained attention at the levels of international competitiveness. Accordingly, a comparative study on the chemical composition of selected Norwegian and French apple ciders was conducted to gain knowledge on what ubiquitous chemical parameters make the Norwegian ciders different from ciders from well-established producing regions. A total of 43 ciders, 24 Norwegian and 19 French, in the category of acidic dominant ciders, were included in the study. Ethanol, individual sugars and organic acids, pH, total phenols, aroma compounds including esters, C6-alcohols, volatile phenols and terpenoids, were analysed. Norwegian ciders showed higher contents in ethanol, malic and citric acids, whereas total phenols, pH, glucose, and fructose were higher in French counterparts. Regarding the aromatic profile, no significant differences were observed for C6-alcohols. In contrast, differences were more expressed in the case of esters and volatile phenols. Norwegian ciders were characterised by higher average concentration for all the groups of esters, with the most important differences measured for higher alcohol acetates. Norwegian ciders also displayed higher contents of 4-vinylphenol and 4-vinylguaiacol while French ciders contained substantially higher levels of 4-ethylphenol and 4-ethylguaiacol. These results are in mutual correlation with the empirical observation reporting Norwegian apple ciders as more acidic, alcoholic and with lighter body but fruitier profile. Whereas French ciders are often perceived with more structure and animalistic profile. Keywords: alcohol, acidity, total phenols, aroma-compounds, apple cider Published in RUNG: 25.03.2024; Views: 1802; Downloads: 6 Link to file This document has many files! More... |
5. Functional characterization of Saccharomyces yeasts from cider produced in HardangerUrban Česnik, Mitja Martelanc, Ingunn Ovsthus, Tatjana Radovanović Vukajlović, Ahmad Hosseini, Branka Mozetič Vodopivec, Lorena Butinar, 2023, original scientific article Keywords: Saccharomyces, Hardanger, characterization, fermentation, cider, non-volatile compounds, volatile organic compounds, partial least squares regression Published in RUNG: 18.09.2023; Views: 1673; Downloads: 9 Full text (4,79 MB) This document has many files! More... |
6. Biogenic amines in Hardanger ciders : the effect of native cider yeasts on biogenic amine productionUrban Česnik, Mitja Martelanc, Branka Mozetič Vodopivec, Ingunn Ovsthus, Lorena Butinar, 2023, published scientific conference contribution abstract Abstract: The presence of bioactive compounds in food and beverages of plant origin is mainly connected with higher nutritional value and better sensory properties. However, some of them can pose a threat to food product quality and human health. For example, excess biogenic amines (BAs) intake can cause different allergenic responses in individuals who have such intolerances.
BAs have been intensively studied in recent years, especially in fermented foods like wine, meat, fish, and cheese. Among fermented beverages is apple cider still rather unexplored from this perspective. Especially since no such data exist for the Norwegian cider. Norwegian cider is becoming more and more popular in Norway in recent years among producers and consumers. Hardanger cider from Western Norway is very different from French, English, or Spanish ciders in terms of sensory characteristics, apple cultivars, and in the fermentation process. In Hardanger, the traditional cider is still produced by spontaneous fermentation of apple juice with naturally occurring yeasts that originate from the fruit or processing equipment surfaces.
Lactic acid bacteria are known to be associated with BA formation. However, several studies reported about the BA-producing yeasts in winemaking. Due to the important role of natural yeasts in the production of Hardanger cider, we focused on the ability of BA formation by native yeasts. Thus, in our study, we followed the amounts of BAs in the Hardanger ciders during the fermentation process and characterize isolated yeasts if they have the ability to produce BAs under cidermaking conditions by performing a micro-fermentation experiment.
From must/cider samples, taken during the fermentation process at 13 producers in the Hardanger region, we isolated 530 yeast isolates. Based on the sequencing of the D1/D2 domain of the 26S rDNA we identified 25 different yeast species. As expected, yeast diversity was higher at the beginning compared to the middle fermentation stage, when mostly different non-Saccharomyces yeast species prevailed, while at the end of fermentation mainly Saccharomyces species with high ethanol tolerance were present.
BAs were analyzed with the HPLC-UV method. In all apple juice/cider samples 4 different BAs (putrescine, cadaverine, histamine, and tyramine) were detected and quantified with external calibration. On average in all cider samples from the producers total BA (summation of all BAs) concentration reached 9,45 mg/L, however in one case even 25 mg/L. Tyramine was the most abundant BA in all fermentation stages. 40 isolated yeast strains were further tested for BA formation in a small-scale experiment by fermenting apple juice. Results show that non-Saccharomyces yeasts mainly form histamine (1,68 mg/L) and tyramine (1,30 mg/L), while Saccharomyces yeasts putrescine (0,48 mg/L) and tyramine (3,53 mg/L). As a general conclusion, the occurrence and distribution of BA concentrations in the small-scale fermentation were lower (2,96 mg/L and 4,01 mg/L) and less variable than in the real ciders (average in final ciders 9,45 mg/L) and with tyramine being the most abundant BA in all samples analyzed. Keywords: cider, yeast, biogenic amines, Hardanger, biodiversity Published in RUNG: 23.06.2023; Views: 1892; Downloads: 7 Link to file This document has many files! More... |
7. Hkratna določitev amino kislin in biogenih aminov v vzorcih jabolčnega soka, ciderja in vina s HPLC-UVMitja Martelanc, Tatjana Radovanović Vukajlović, Urban Česnik, Lorena Butinar, Melita Sternad Lemut, Branka Mozetič Vodopivec, 2022, published scientific conference contribution Keywords: Cider, vino, jabolčni sok, HPLC-UV, Amino kisline, biogeni amini. Published in RUNG: 20.02.2023; Views: 2764; Downloads: 0 This document has many files! More... |
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9. Cider yeasts associated with Hardanger cider during fermentation processUrban Česnik, Mitja Martelanc, Branka Mozetič Vodopivec, Ingunn Ovsthus, Lorena Butinar, 2022, published scientific conference contribution abstract Abstract: In the Hardanger area in Western Norway, the production of cider has a long tradition that goes back to the 12th century, when monks introduced apple growing in this area. Nowadays, this is also the main area of fruit production in Norway. Despite the strict regulation of the alcoholic beverage production in Norway, traditional cider is still produced on some farms in this area. Therefore, our aim was to study the ecology and biodiversity of the yeasts associated with the cider production in the Hardanger area during fermentation process; especially of traditional cider, which is produced by a spontaneous fermentation of apple juice, performed by naturally occurring indigenous yeasts that originate from the fruit or the surfaces of the processing equipment.
In our study, samples of fermenting juice/cider were taken during fermentation process from 12 producers, located in 12 different locations in Hardanger region. Classical cultivation methods using WL (Wallerstein Laboratories) agar medium with added chloramphenicol enable us to isolate a total of 530 yeast isolates that were stored in in-house yeast collection at the NIBIO and included also at the Wine Research Centre collection. Based on the sequencing of the D1/D2 domain of the 26S rDNA we managed to identify 357 isolates and distinguished 27 different yeast species as follows: Aureobasidium pullulans, Candida californica, C. oleophila, C, sake, Hanseniaspora meyeri, H. uvarum, H. valbyensis. Kregervanrija fluxuum, Kregervanrija sp., Metschnikowia andauensis, M. chrysoperlae, M. fructicola, M. pulcherrima, Metschnikowia sp, Pichia fermentans, P. kluyveri, P. membranifaciens, P. nakasei, Piskurozyma capsuligena, Rhodotorula nothofagi, Saccharomyces bayanus, S. cerevisiae, S. paradoxus, S. pastorianus, Saccharomyces sp., S. uvarum and Torulaspora delbrueckii.
Even though we were not able to obtain samples in three different fermentation stages (beginning, middle and at the end of fermentation) from all producers, we could observe yeast succession during fermentation progress. Yeast diversity was higher at the beginning comparing to the middle of fermentation, when mostly different non-Saccharomyces yeast species prevailed, while in the middle of fermentation 11 species were detected (Candida californica, H. uvarum, H. valbyensis, Kregervanrija sp., K. fluxuum, Pichia membranifaciens, Metschnikowia pulcherrima, Saccharomyces sp, S. bayanus, S. uvarum and S. cerevisie). On the other hand, at the end of fermentation mainly Saccharomyces species with high ethanol tolerance were present (Saccharomyces sp., S. cerevisiae, bayanus, S. uvarum and P. fermentans).
In samples that were collected from three producers in all three fermentation stages also quality parameters were determined (ethanol, organic acids, sugars, biogenic amines) with in-house developed methods using HPLC-UV/RID. The most important sugars in ciders were fructose and glucose, as expected. Two producers added sugar to increase the level of ethanol in the middle of fermentation, which is a common procedure in the Hardanger area. Ethanol and organic acid analysis indicated that fermentations went in the right direction, since all parameters were within normal limits. Including the acetic acid level, an indicator of low cider quality, was very low (average around 0,06 g/L). The alcohol incised from the beginning to end fermentation in all samples analysed and minimum concentration was 2,71 g/L. In ciders we detected four biogenic amines (putrescin,
cadaverine, histamine and tyramine). The average amount was 32 mg/L and the most abundant was tyramine. Keywords: indigenous yeasts, biodiversity, spontaneous fermentation, cider-making Published in RUNG: 18.10.2022; Views: 2395; Downloads: 0 This document has many files! More... |
10. Validacija HPLC metode za določanje organskih kislin in sladkorjev v vinuKristjan Mesar, 2022, undergraduate thesis Abstract: V tem diplomskem delu smo obravnavali proces validacije dveh analiznih metod za določanje kakovosti vina. In sicer smo določali organske kisline kot so vinska kislina, jabolčna kislina, mlečna kislina, citronska kislina, ocetna kislina, in monosaharide glukozo in fruktozo. Analiza teh parametrov je pomembna pri nadzoru procesa pridelave vina kot tudi spremljanju kakovosti vina. V diplomski nalogi smo pregledali glavne metode za določanje kislin in sladkorjev v grozdju in vinu, v samem poskusu pa smo uporabili kromatografsko tehniko na osnovi tekočinske kromatografijo visoke ločljivosti v kombinaciji z detekcijo v ultravijoličnem (UV) za organske kisline in z detekcijo lomnega količnika (RID) za monosaharidne sladkorje. HPLC-UV/RID sistem omogoča enostavno pripravo vzorca na analizo (samo filtracija, mogoče redčitev, če je potrebna), hitro analizo in omogoča zadovoljive meje detekcije za potrebe določevanja omenjenih parametrov. Obe metodi smo opisali z različnimi parametri, kot je ponovljivost, linearnost, izkoristek v štirih različnih matriksih (dve beli vini, eno rdeče in eno jabolčno vino – cider), meja detekcije in meja kvantifikacije. Rezultate kvantifikacije skupnih sladkorjev in organskih kislin v vzorcih vin smo primerjali z rezultati akreditiranega laboratorija kjer je bilo to mogoče in ugotovili, da kvantifikacija z metodo standardnega dodatka omogoča primerljive rezultate z akreditiranim laboratorijem, predvsem za parameter skupni fermentirani sladkorji (glukoza in fruktoza). Vsebnost skupnih kislin določenih kromatografsko je bila nekoliko manjša od parametra skupne titrabilne kisline, kar je najverjetneje posledica manjšega števila posameznih kislin, ki smo jih vključili v ta parameter, pa tudi drugačne metode določanja kislin. Keywords: vino, cider, HPLC, UV, RID, organske kisline, sladkorji, validacija Published in RUNG: 02.08.2022; Views: 2836; Downloads: 68 Full text (1,10 MB) |