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2. WATER-POLYPEPTIDE INTERACTION IN CLASSICAL MODELS OF HELIX-COIL TRANSITIONKnarik Yeritsyan, 2016, undergraduate thesis Abstract: Zimm-Bragg model is the simplest to describe the conformational transitions in biopolymers and is regularly used for preocessing the experimental data. We review the model and its Hamiltonian definition with the goal to introduce the interaction with water into the picture. We show how modified ZB model with the account of water-polypeptide interactions allows to describe both cold denaturation and helix-coil transition and derive such the formula explicitly. The obtained theoretical expression for the helicity degree contains two independent parameters that can be fitted with the experimental data to determine the parameters of cold denaturation and helix-coil transition from a single fit and for a single set of experimental data.
Found in: ključnih besedah
Keywords: Zimm-Bragg model, conformational transitions in biopolymers, cold denaturation
Published: 28.09.2016; Views: 4582; Downloads: 193
 Fulltext (1,70 MB) |
3. Phenolic Contents and Postharvest Quality Changes of Norwegian ‘Mallard’ plums (Prunus domestica L.) as a Consequence of Delayed Time to Low Temperature StorageEivind Vangdal, Alena Gibalova, Branka Mozetič Vodopivec, 2012, published scientific conference contribution Abstract: During the picking season in the Norwegian plum production areas, the
harvested plums are brought to cold storage in packing houses once or twice a day.
Plums picked late in the afternoon may even not be cooled properly until the next
day. The average time from picking until the plums are placed in a cold storage is
approximately 8 hours. Previous reports have shown that delayed cooling of sweet
cherries and apples reduced the fruits’ susceptibility to physiological or fungal
decay. An experiment was performed in order to evaluate the impact of different
delay-to-cold storage times (0, 5, 10, 15 and 25 hours) on quality and phenolic
contents changes of Norwegian ‘Mallard’ plums during cold storage, transport, and
retail in normal atmosphere (NA). The fruits (10 fruits in PE tray, n=3) were stored
for 14 days at 1°C, followed by 3 days at 7°C and then for 4 days at 20°C. Fruit
quality parameters (firmness, colour in CIE L, a*, b* colour space, weight, rot
occurrence (%)) and total phenol and anthocyanin content were determined.
Samples were analyzed just before cold storage and then after 14 days at 1°C, 3 days
at 7°C, or 4 days at 20°C. Evaluation of results has shown that delay of cooling has a
retarding effect on rot development during 20°C storage (post cold transport),
weight loss and stimulates anthocyanin accumulation and colour changes to some
extent. At the end of storage no effect on fruit firmness could be observed.
Found in: ključnih besedah
Keywords: anthocyanins, total phenols, colour, rot, firmness, postharvest, plums, cold
storage
Published: 11.11.2016; Views: 2880; Downloads: 0
Fulltext (268,22 KB) |
4. Developments of a new mirror technology for the Cherenkov Telescope ArrayMarko Zavrtanik, Danilo Zavrtanik, Gabrijela Zaharijas, Lili Yang, Serguei Vorobiov, Samo Stanič, Gašper Kukec Mezek, Andrej Filipčič, Jerzy Michałowski, 2015, published scientific conference contribution Found in: ključnih besedah
Keywords: Cherenkov Telescope Array (CTA), mirror technology for CTA, cold-slumping, front reflecting aluminium-coated panel
Published: 16.01.2017; Views: 2142; Downloads: 183
Fulltext (303,93 KB) |
5. Implicit water model within the Zimm-Bragg approach to analyze experimental data for heat and cold denaturation of proteinsJože Grdadolnik, Matjaž Valant, Sh. A. Tonoyan, Artem Badasyan, 2021, original scientific article Abstract: Studies of biopolymer conformations essentially rely on theoretical models that are routinely
used to process and analyze experimental data. While modern experiments allow study of
single molecules in vivo, corresponding theories date back to the early 1950s and require an
essential update to include the recent significant progress in the description of water. The
Hamiltonian formulation of the Zimm-Bragg model we propose includes a simplified, yet
explicit model of water-polypeptide interactions that transforms into the equivalent implicit
description after performing the summation of solvent degrees of freedom in the partition
function. Here we show that our model fits very well to the circular dichroism experimental
data for both heat and cold denaturation and provides the energies of inter- and intra-
molecular H-bonds, unavailable with other processing methods. The revealed delicate
balance between these energies determines the conditions for the existence of cold dena-
turation and thus clarifies its absence in some proteins.
Found in: ključnih besedah
Keywords: protein folding, cold denaturation, water, Zimm-Bragg model
Published: 06.05.2021; Views: 409; Downloads: 2
 Fulltext (0,00 KB)
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