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1.
Engineering of functional nanosnowflakes from gold nanocarriers capped with amino-modified DNA oligonucleotides
Alexandre Loukanov, Velichka Arahangelova, Saim Emin, Chavdar Filipov, 2023, original scientific article

Abstract: Abstract The design, engineering and electron microscopic characterization of anisotropic nanosized snowflake‐like structural assemblies (nanosnowflakes) is reported. They were fabricated through immobilization of double stranded amine‐modified and thiol‐terminated DNA oligonucleotides on the surface of ultra‐small isotropic gold nanoparticles used as nanocarriers. The transmission electron microscopy images combined with spectrophotometric data revealed the formation of self‐assembled structural aggregation between individual ligands‐coated nanoparticles. They act as seeds for the further spontaneous dendritic growth in different directions. Their anisotropic morphology is formed due to the occurrence of facilitated electrostatic interactions between positive charged amino‐groups and the negative sugar‐phosphate backbone of oligonucleotides. Thus, nanosnowflakes with size distribution between 40 and 80 nm were obtained. The microscopic analysis demonstrated also that the stable nanosnowflakes structure was highly dependent on the solution ionic strength, which effect the charge fluctuation within the assembly. The reported DNA functionalized nanostructures have potential to be applied as a platform for development of therapeutic materials, as well as drug delivery nanosystems. Research Highlights The engineering, fabrication, and microscopic characterization of DNA nanosnowflakes is reported. The electron microscopy analysis revealed formation of self‐assemblies with anisotropic morphology. The nanosnowflakes size distribution was between 40 and 80 nm.
Keywords: DNA, nanocarrier, functionalization, drug delivery
Published in RUNG: 18.03.2024; Views: 418; Downloads: 0
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2.
Combining panning design and expression options allows obtaining nanobodies trimmed for specific applications
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, panning, functionalization
Published in RUNG: 15.05.2023; Views: 1021; Downloads: 0
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3.
Recombinant ligands as tunable biotechnological tools
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, protein functionalization, biosensors
Published in RUNG: 17.05.2022; Views: 1575; Downloads: 0
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4.
Opportunities offered by recombinant ligands
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, protein functionalization, rational mutagenesis
Published in RUNG: 13.05.2022; Views: 1679; Downloads: 0
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Pre-immune libraries of recombinant nanobodies: outcome from 15-year experience
Ario De Marco, invited lecture at foreign university

Keywords: Nanobodies, in vitro panning, protein functionalization
Published in RUNG: 14.02.2020; Views: 2869; Downloads: 0
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8.
Nanobodies as a flexible biotechnological platform
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, phage display, crystallography chaperones, functionalization strategies
Published in RUNG: 10.11.2019; Views: 3000; Downloads: 0
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9.
Combining in vitro panning of nanobody pre-immune libraries with sequence (rational) engineering to get application-tailored reagents
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, extracellular vesicles, functionalization tags
Published in RUNG: 10.09.2019; Views: 2930; Downloads: 0
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