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Found in: ključnih besedah
Keywords: cell receptor, DNA nanostructures, cell membrane nanoenvironment
Published: 05.11.2020; Views: 2103; Downloads: 0
Fulltext (16,22 MB)

Found in: ključnih besedah
Keywords: graphene, membrane, chitosan, polysaccharide, fuel cell, alkaline, ethanol, morphology, elemental analysis
Published: 03.12.2020; Views: 1859; Downloads: 0
Fulltext (19,13 MB)

Abstract: Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Found in: ključnih besedah
Summary of found: ...nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality...
Keywords: nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Published: 19.04.2017; Views: 4785; Downloads: 0
Fulltext (1,11 MB)

Found in: ključnih besedah
Summary of found: ...ce nucleation, Airborne bacteria, Aeromicrobiology, Bioprecipitation, Outer membrane vesicles...
Keywords: INA bacteria, Ice nucleation, Airborne bacteria, Aeromicrobiology, Bioprecipitation, Outer membrane vesicles
Published: 04.01.2021; Views: 1454; Downloads: 0
Fulltext (3,10 MB)

Abstract: Copper is a trace element vital to many cellular functions. Yet its abnormal levels are toxic to cells, provoking a variety of severe diseases. The high affinity copper transporter 1 (CTR1), being the main in-cell copper [Cu(I)] entry route, tightly regulates its cellular uptake via a still elusive mechanism. Here, all-atoms simulations unlock the molecular terms of Cu(I) transport in eukaryotes disclosing that the two methionine (Met) triads, forming the selectivity filter, play an unprecedented dual role both enabling selective Cu(I) transport and regulating its uptake rate thanks to an intimate coupling between the conformational plasticity of their bulky side chains and the number of bound Cu(I) ions. Namely, the Met residues act as a gate reducing the Cu(I) import rate when two ions simultaneously bind to CTR1. This may represent an elegant autoregulatory mechanism through which CTR1 protects the cells from excessively high, and hence toxic, in-cell Cu(I) levels. Overall, our outcomes resolve fundamental questions in CTR1 biology and open new windows of opportunity to tackle diseases associated with an imbalanced copper uptake.
Found in: ključnih besedah
Summary of found: ...copper, membrane transporter, molecular dynamics, QM/MM, free energy...
Keywords: copper, membrane transporter, molecular dynamics, QM/MM, free energy
Published: 15.09.2022; Views: 346; Downloads: 0
Fulltext (3,24 MB)