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3. Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertationGbenga Folorunsho Oginni, 2024, doctoral dissertation Abstract: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Keywords: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Published in RUNG: 10.09.2024; Views: 619; Downloads: 11
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4. Urinary extracellular vesicles as a readily available biomarker source : a simplified stratification methodLidija Filipović, Milica Spasojević Savković, Radivoje Prodanović, Suzana Matijašević Joković, Sanja Stevanović, Ario De Marco, Maja Kosanović, Goran Brajušković, Milica Popovic, 2024, original scientific article Keywords: nanobodies, extracellular vesicles, immunoaffinity purification
Published in RUNG: 23.07.2024; Views: 952; Downloads: 8
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6. Novel bispecific nanobody mitigates experimental intestinal inflammation in mice by targeting TNF-▫$\alpha$▫ and IL-23p19 bioactivitiesJiewen Wang, Guangbo Kang, Huiying Lu, Ario De Marco, Haibin Yuan, Zelin Feng, Mengxue Gao, Xiaoli Wang, Huahong Wang, Xiaolan Zhang, 2024, original scientific article Keywords: bispecific constructs, nanobodies, intestinal inflammation
Published in RUNG: 27.03.2024; Views: 1591; Downloads: 7
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