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2.
Combining panning design and expression options allows obtaining nanobodies trimmed for specific applications
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, panning, functionalization
Published in RUNG: 15.05.2023; Views: 997; Downloads: 0
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3.
Nanobodies isolated from pre-immune libraries as tunable biotechnological tools
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, panning, modeling
Published in RUNG: 27.02.2020; Views: 2520; Downloads: 0
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4.
Pre-immune libraries of recombinant nanobodies: outcome from 15-year experience
Ario De Marco, invited lecture at foreign university

Keywords: Nanobodies, in vitro panning, protein functionalization
Published in RUNG: 14.02.2020; Views: 2851; Downloads: 0
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5.
Nanobodies: How to Exploit Pre-Immune Libraries and Recombinant Antibody Fragments
Ario De Marco, unpublished invited conference lecture

Keywords: nanobodies, in vitro panning, phage display
Published in RUNG: 23.08.2019; Views: 3212; Downloads: 0
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6.
In vitro and in silico approaches to obtain tailored reagents – Nanobodies as model
Ario De Marco, invited lecture at foreign university

Keywords: Immunoreagents, nanobodies, in vitro panning, recombinant antibody production
Published in RUNG: 21.09.2018; Views: 3354; Downloads: 0
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7.
Pre-immune nanobody collections and their biotechnological applications
Ario De Marco, invited lecture at foreign university

Keywords: phage libraries, nanobodies, in vitro antibody panning
Published in RUNG: 17.09.2018; Views: 3194; Downloads: 0
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8.
Nanobodies: a flexible technological platform
Ario De Marco, invited lecture at foreign university

Keywords: Nanobodies, immunoreagents, recombinant antibody panning
Published in RUNG: 17.09.2018; Views: 3328; Downloads: 0
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9.
Nanobodies: flexible structures for biotechnological applications
Ario De Marco, unpublished invited conference lecture

Keywords: nanobodies, in vitro panning, extracellular vesicles, oncology, toxic algae
Published in RUNG: 16.03.2018; Views: 3771; Downloads: 0
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10.
Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Ronan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, Francois Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Pérez, Nicolae Ghinea, Ario De Marco, 2017, original scientific article

Abstract: Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Keywords: nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Published in RUNG: 19.10.2017; Views: 4079; Downloads: 0
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