1. Effective microorganisms technology applied to sewage sludge and tested in short exposure on Lepidium sativumTanja Buh, Leja Goljat, Darian Rampih, Petra Makorič, Sara Pignattelli, 2024, original scientific article Abstract: Sewage sludge has fertilizer properties and can supply a large amount of necessary nutrients to the crops, because it is full of
organic matter, carbon, nitrogen and other nutrients, but on the other hand, it also contains a lot of toxic compounds, derived
from its origin, such as heavy metals, antibiotics and microplastics. Effective microorganisms are a collection of naturally
occurring beneficial microorganisms that are able to coexist and are commonly used in agriculture and gardening to improve
plant performance and production. In this study, increasing concentrations of sewage sludge alone and added with effective
microorganisms were evaluated in a short exposure on Lepidium sativum L. Parameters that were evaluated are: (i) percentage
inhibition of germination, (ii) root length, (iii) biomass, (iv) soil pH, (v) total organic carbon and nitrogen both at soil and
at root level. Results carried out from our experiment highlighted that effective microorganisms when coupled with sludge
are able to restore biometric parameters by resetting seeds germinability inhibition and improving root elongation more than
50% when compared with plants added only with sludge, restoring the values almost of those to the control plants, as well
as for soil pH values. Total organic carbon and total nitrogen are boosted at soil level almost at 50% when compared with
the same concentrations added only with sludge, while at root level they appear decreased only in plants directly added with
sludge treated with effective microorganisms Keywords: sewage sludge, effective microorganism, total organic carbon, total nitrogen, germinability, short plants exposure, acute toxicity, biomass, pH Published in RUNG: 12.04.2024; Views: 1421; Downloads: 3 Link to file This document has many files! More... |
2. Selenium affects mercury ligand environment in terrestrial food chain – a XAS studyKatarina Vogel Mikuš, Alojz Kodre, Iztok Arčon, Anja Kavčič, 2022, published scientific conference contribution abstract Abstract: Selenium (Se) supplied in inorganic form (as selenate or selenite) was shown to decrease mercury (Hg) toxicity by forming HgSe in soils as well as in animal and human tissues, while for plants there is no evidence of Hg-Se complexation. Although Se in not an essential element for plants it was shown to counteract various abiotic stresses when applied at trace amounts. The aim of this work was therefore to study physiological responses and Hg speciation in plant/ fungi-animal food chain. Lettuce (Lactuca sativa) and porcini mushrooms (Boletus edulis) were taken as model plant/ fungal species and Spanish slug (Arion vulgaris) as a model animal species. The plants, fed to the slugs, were grown in HgCl2 contaminated soil or soil from the vicinity of Hg mine in Idrija with traces of HgS and methyl Hg). Physiological parameters of plants and slugs were monitored during the experiment. At the end the biological material was frozen in LN2 and freeze dried. Hg L3-edge (12284 eV) XANES and EXAFS spectra of the biological samples and standards were measured at liquid helium temperature in fluorescence detection mode at the BM30B beamline of the ESRF synchrotron in Grenoble, using the 30-segment germanium solid state detector [1]. The results showed that addition of Se alleviated Hg toxic effects in the food chain started at HgCl2-contaminated soil, while for the soil from Idrija, containing low amounts of highly toxic methyl-Hg, the beneficial effect was less prominent [2]. No Hg-Se complexes were detected in plants, while in mushrooms and slugs the complexation was confirmed. Addition of Se to the plants, however, changed Hg ligand environment in plant tissues from sulphur to nitrogen ligands. Hg and Se both target the -SH functional groups in the plant tissues, so toxic effects of Hg are rather enhanced than alleviated by addition of Se. Nevertheless, the addition of Se to the plants is beneficial for higher trophic levels and lowers Hg toxicity for the primary consumers, the slugs. Keywords: mercury, toxicity, ligand environment, XANES, EXAFS, food chain, plant, slug, fungi Published in RUNG: 05.12.2022; Views: 2277; Downloads: 0 This document has many files! More... |
3. Impact of microplastics on growth, photosynthesis and essential elements in Cucurbita pepo L.Ilaria Colzi, Luciana Renna, Elisabetta Bianchi, Maria Beatrice Castellani, Andrea Coppi, Sara Pignattelli, Stefano Loppi, Cristina Gonnelli, 2022, original scientific article Abstract: In this study, Cucurbita pepo L., one of the most cultivated, consumed and economically important crop worldwide, was used as model plant to test the toxic effects of the four most abundant microplastics identified in contaminated soils, i.e. polypropylene (PP), polyethylene (PE), polyvinylchloride (PVC), and polyethyleneterephthalate (PET). Cucurbita plants were grown in pots with increasing concentrations of the microplastics, then plant biometry, photosynthetic parameters and ionome of treated vs. untreated samples were compared to evaluate the toxicity of each plastic. All the pollutants impaired root and, especially, shoot growth. Specific and concentration-dependant effects of the different microplastics were found, including reduction in leaf size, chlorophyll content and photosynthetic efficiency, as well as changes in the micro- and macro-elemental profile. Among all the microplastics, PVC was identified as the most toxic and PE as the less toxic material. PVC decreased the dimensions of the leaf lamina, the values of the photosynthetic performance index and the plant iron concentration to a higher extent in respect to the other treatments. Microplastic toxicity exerted on the growth of C. pepo raises concerns about possible yield and economic loss, as well as for risks of a possible transfer into the food chain. Keywords: microplastic toxicity, leaf traits, chlorophyll fluorescence, ionome, zucchini Published in RUNG: 17.12.2021; Views: 2965; Downloads: 8 Link to full text This document has many files! More... |
4. Arsenic in natural waters: hydrogeochemistry characterization and toxicity effectsDoroteja Gošar, 2018, master's thesis Abstract: Arsenic contamination in natural water is a worldwide problem and a major health concern. In master thesis hydrogeochemistry and toxicity effects of natural waters rich in arsenic were studied. The main objective of the master thesis was to evaluate As pollution of the Freixeda stream and groundwater in abandoned Freixeda gold mine area in NE Portugal near Mirandela city (41.413767 N 7.103562 W) and compare it with the data from previous studies. Further on, toxicological evaluation of selected water samples was performed in the in vitro system of human cell line Caco-2.
Chemical analyses of sampled water samples with use of different modelling sofware show that groundwater have higher sulphate and bicarbonate values than surface water, which could be the reason for As desorption and higher As values in groundwater. Water-rock interaction promotes reduction and dissolution of sulphide minerals and in reductive environments dissolution of secondary Fe minerals releases adsorbed As into solution.
Toxicological testing on human cells included cytotoxicity assay, genotoxicity assay and production of reactive oxigene species (ROS). Genotoxicity was only modestly affected by a short-term exposure to As-contaminated water samples, however, higher concentrations of As in real samples lead to higher level of oxidative stress and decreased cell viability. Exposure of cells to pure As(III) solution show clear concentration dependent decrease in cell metabolism and viability, strong genotoxicity and increased ROS generation.
Considering the worldwide extent of As contamination in natural waters and ability of intestinal epithelium to reduce the potential harmful effects of As, more studies evaluating the human intestinal permeability for As should be done in the future. Keywords: Water quality, arsenic, hydrogeochemistry, toxicity, Caco-2 cell line Published in RUNG: 26.09.2018; Views: 6731; Downloads: 209 Full text (3,76 MB) |
5. PHOTOLYSIS AND ECOTOXICITY OF THE ANTIBIOTIC CIPROFLOXACINKetevan Neparidze, 2017, undergraduate thesis Abstract: Over the last few decades, numerous pharmaceuticals coming from domestic sewage, livestock, hospitals and industries have been determined in ground, surface and drinking waters. A common example of these pollutants are fluoroquinolones, which are powerful antibiotics used in human and veterinary medicine. The presence of fluoroquinolones in the environment even at low concentration constitutes a threat to ecosystems and human health because they can lead to bacterial resistance issues. Up to now the environmental impacts of fluoroquinolones and their degradation products are not fully understood but it is known that they can negatively affect plants and aquatic organisms. In this study the degradation of ciprofloxacin (CIP) present in synthetic freshwater was investigated under simulated sunlight. Concentrations before and after degradation were determined using HPLC-DAD. The mineralisation rate of the treated solution was monitored on a total organic carbon analyser. The ecotoxicity of the initial samples and the degradation mixtures was assessed on organisms belonging to different trophic levels: a marine bacterium (Vibrio fischeri), a freshwater invertebrate (Daphnia magna) and seed clover (Trifolium repens). In parallel, the EC50 of CIP was determined for all organisms. The Vibrio fischeri and Trifolium repens tests showed that EC50 value was more than 250 mg/L and for Daphnia magna was 190 mg/L. Photolysis of CIP showed that after 90 min of irradiation, CIP was almost totally degraded (99%). Photolysis process of CIP led to the formation of toxic by-products towards Vibrio fischeri. The toxicity test results on Trifolium repens did not show significant difference between the control and the treated plants. Thus, CIP and its degradation products have no effect on the germination or root length of Trifolium repens. Tests on Daphnia magna after 240 min irradiation showed a 94% reduction of the toxic effect. Non-purgeable organic carbon (NPOC) analysis showed that there is no significant mineralisation of the sample even after 240 min of irradiation. Keywords: Key words: fluoroquinolones, ciprofloxacin, photolysis, toxicity tests, EC50 determination Published in RUNG: 31.01.2017; Views: 6819; Downloads: 318 Full text (947,78 KB) |
6. Arbuscular mycorrhizal fungi alter Hg root uptake and ligand environment as studied by X-ray absorption fine structureAlojz Kodre, Iztok Arčon, Marta Debeljak, Mateja Potisek, Matevž Likar, Katarina Vogel-Mikuš, 2017, original scientific article Abstract: Mercury (Hg) – plant – fungal interactions are only poorly studied. Hg speciation and ligand environment
in maize roots inoculated with arbuscular mycorrhizal (AM) fungi were investigated in order to better
understand the role of AM in Hg soil to root transfer.
The maize plants were grown in Hg polluted substrate (50 mg g1 as dissolved HgCl2) and inoculated
with AM fungi originating from: a) highly Hg polluted environment of a former Hg smelting site in Idrija,
Slovenia, (Glomus sp. – sample AmI), and b) non-polluted environment (commercial AM inoculum
Symbivit1 – sample AmC). Hg speciation and ligand environment in maize roots was studied by Hg-L3
XANES and EXAFS with emphasis on XAS methodology – modelling and fitting the XAFS spectra to extract in a reliable way as much information on Hg coordination as possible. The AmI plants developed more arbuscules and less vesicles than the AmC plants, and also
accumulated more Hg in the roots. A clear difference in Hg coordination between the AM (AmC & AmI)
and the control (ConC & ConI) plants is recognized in Hg L3-edge EXAFS analysis: in the ConC & ConI
maize roots 73–80% of Hg is attached between two sulphur atoms at the distance of 2.34 Å. The remaining
ligand is nitrogen at 2.04 Å. In AmI & AmC roots another Hg-S attachment encompassing four thiol groups
at the S-distance of 2.50 Å are identified, accounting for 21–26%. AM fungi can modify Hg ligand environment in plant roots, thus playing an important role in biogeochemical cycling of Hg in terrestrial
ecosystems. Keywords: EXAFS
XANES
Arbuscular mycorrhiza
Phytoremediation
Toxicity
Hg coordination
Ligand environment Published in RUNG: 27.09.2016; Views: 7339; Downloads: 0 This document has many files! More... |
7. Comparative analysis of UV-C/H2O2 and UV-A/TiO2 processes for the degradation of diclofenac in waterDaria Juretic Perisic, Marin Kovacic, Hrvoje Kušić, Urška Lavrenčič Štangar, Vedrana Marin, Ana Lončarić Božić, 2016, original scientific article Abstract: The study investigates the treatment of diclofenac (DCF), a pharmaceutical
included in the first watch list of the European Water Framework Directive
as a new potential priority substance in water. Since the conventional wastewater
treatment technologies do not efficiently remove DCF, advanced treatment technologies
capable of its complete removal or destruction of its biological activity,
need to be evaluated and eventually employed. For that purpose, typical representatives
of photooxidative and photocatalytic advanced oxidation processes were
applied. The effectiveness of UV-C/H2O2 and UV-A/TiO2 were compared regarding
DCF conversion and mineralization kinetics, water quality parameters for
assessing biodegradability and toxicity. In spite of similar biodegradability profiles,
the obtained results indicate different DCF degradation pathways, which are
reflected in different profiles of toxicity towards Vibrio fischeri. The observed DCF
conversion and mineralization kinetics revealed the benefits of UV-C/H2O2 process.
However, lower toxicity favored the application of photocatalytic over photooxidative
treatment for DCF removal. Keywords: Diclofenac, Photooxidation, Photocatalysis, Biodegradability, Toxicity Published in RUNG: 21.07.2016; Views: 5802; Downloads: 0 This document has many files! More... |
8. Diclofenac removal by simulated solar assisted photocatalysis using TiO2-based zeolite catalyst; mechanisms, pathways and environmental aspectsSubhan Salaeh, Daria Juretic Perisic, Martina Biosic, Hrvoje Kušić, Sandra Babich, Urška Lavrenčič Štangar, Dionysios D. Dionysiou, Ana Lončarić Božić, 2016, original scientific article Abstract: The study explores the potential of immobilized TiO2-based zeolite composite photocatalyst (TiO2-FeZ)
made of commercial AEROXIDE TiO2 P25 and iron-exchanged zeolite of ZSM5 type (FeZ), for solar assisted
treatment of diclofenac (DCF), pharmaceutical included in the ‘‘watch list” during last prioritization in
water legislation by EU.
In this study the efficiency of applied photocatalytic treatment, solar/TiO2-FeZ/H2O2, of DCF water
solution was evaluated on basis of DCF removal and conversion kinetics, as well as the changes of
common parameters for assessing water quality. Hence, the changes in the removal and mineralization
of overall organic content, biodegradability, toxicity to Vibrio fischeri, dechlorination of DCF and
its formed by-products, were monitored during the treatment. The obtained data were correlated with
the evolution of DCF by-products, identified and monitored during the treatment by HPLC/MSMS
analysis. In order to estimate the influence of water matrix, all experiments were performed in the
presence of chloride or sulphate as counter ions. The obtained data revealed that degradation
mechanism of DCF by applied treatment process using immobilized TiO2-FeZ includes the adsorption
onto photocatalyst surface and consequent degradation. The contribution of homogeneous Fenton
reaction due to leached iron ions was found to be negligible. The adsorption and degradation pathway of DCF were influenced by the type of counter ions, which was reflected in the observed changes of
water quality parameters. Keywords: Solar photocatalysis, TiO2-FeZ catalyst, Diclofenac, Degradation pathway, Biodegradability, Toxicity Published in RUNG: 21.07.2016; Views: 6583; Downloads: 0 This document has many files! More... |
9. Removal of diclofenac from water by zeolite-assisted advanced oxidation processesDaria Juretic Perisic, Vanja Gilja, Mirjana Novak Stankov, Zvonimir Katancic, Hrvoje Kusic, Urška Lavrenčič Štangar, Dionysios D. Dionysiou, Ana Loncaric Bozic, 2016, original scientific article Keywords: Diclofenac, FeZSM5, Biodegradability, Toxicity, Catalyst stability Published in RUNG: 24.02.2016; Views: 6005; Downloads: 0 This document has many files! More... |
10. Stability and toxicity of selected chlorinated benzophenone-type UV filters in watersPolonca Trebše, Darko Dolenc, Romina Žabar, Tatjana Tišler, Gorica Grbović, Jun Yao, Rensheng Zhuang, 2013, original scientific article Abstract: Stability and toxicity of selected chlorinated benzophenone-type UV filters in waters Keywords: UV filter, photostability, chlorination, toxicity, Vibrio fischeri Published in RUNG: 05.01.2016; Views: 7149; Downloads: 1 |