1. Regenerated cellulose films with chitosan and polyvinyl alcohol: Effect of the moisture content on the barrier, mechanical and optical propertiesGonzalo Velazquez, Manuel Vazquez, Patricia Cazón, 2020, original scientific article Abstract: The aim of this research was to evaluate the effect of moisture content on the mechanical, barrier and optical properties of films obtained from regenerated cellulose with chitosan and polyvinyl alcohol equilibrated at several relative humidity conditions. The experimental moisture adsorption isotherms were fitted using the Guggenheim-Anderson-DeBoer model. The adsorption isotherm showed a typical type II sigmoidal shape. The highest moisture content (27.53 %) was obtained at a water activity of 0.9. The water vapour permeability values increased up to 6.34·10−11 g/ m s Pa as the moisture content of the films increased. Tensile strength, percentage of elongation, Young’s modulus, burst strength and distance to burst showed a significant plasticizing effect of the water molecules. Results suggest that interactions between film components and water molecules decrease the transmittance in the UV region and the transparency. Consequently, water molecules improve the UV-barrier properties of the films and increasing the opacity. Found in: ključnih besedah Summary of found: ...content (27.53 %) was obtained at a water activity of 0.9. The water vapour permeability... Keywords: Adsorption isotherms, Plasticization, Regenerated cellulose, Chitosan, Polyvinyl alcohol, Water vapour permeability, Moisture content Published: 09.12.2020; Views: 1897; Downloads: 0
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4. Biological role of Grapevine fanleaf virus (GFLV) in winegrowing region of Northern PrimorskaAnastazija Jež Krebelj, 2015, doctoral dissertation Abstract: Grapevines (Vitis vinifera L.) represent one of the most important crops in the world in terms of both production and economic importance. Grapevines are exposed to many types of abiotic stresses (e.g., drought, flooding, low and high temperature, salinity) and biotic stresses (e.g., viruses, bacteria, phytoplasma, fungal disease) during their life-cycle. Therefore, grapevines elicit the appropriate defence mechanisms.
In the first part of this study, we monitored the occurrence of Grapevine fanleaf virus (GFLV) infection, which causes progressive decline of infected grapevines and lowers their yield. Grapevines were also tested for the presence of other viruses important for grapevines: Arabis mosaic virus (ArMV), Grapevine leafroll associated virus (GLRaV)-1, -2, -3, -4, -9, Grapevine virus A (GVA), Grapevine fleck virus (GFkV) in this study; and by Cigoj (2015): Grapevine virus B (GVB), Tomato black ring virus (TBRV), Grapevine chrome mosaic virus (GCMV), Tomato ringspot virus (ToRSV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV), and Tobacco ringspot virus (TRSV). Using ELISA, the presence of the following grapevine viruses were detected: GFLV, (GFkV), (GVA), and Grapevine leafroll associated viruses- 1, -2, -3,.
A wide range of GFLV symptoms caused by grapevine fanleaf disease in naturally infected vineyards were observed, including leaf, shoot and cluster malformations and leaf yellowing. GFLV is disseminated by its biological vector X. index, and through vegetative propagation of virus-infected material. The spread of GFLV in the vineyards was investigated here.
We constructed a spatio-temporal study of the GFLV titres during the seasons and throughout the grapevine, for its distribution in different grapevine organs through the season. This study shows that young leaves have high virus titres through the whole vegetative period, while mature leaves, tendrils and flower/ berry clusters only have high titres at the beginning of the vegetative period. The seeds retain high virus titres after berry colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with an increase outside and at the beginning of the vegetative period. In flower/ berry clusters, mature leaves and tendrils, the GFLV titres decrease significantly over the vegetative period. Additionally, different GFLV titres were shown in five different cultivars, and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptom appearance and GFLV titres was analysed.
Grapevines adapt to abiotic stresses and biotic stresses by the expression of a wide range of stress-responsive genes, which are thought to have key roles in stress tolerance and survival.
SWP of the infected grapevines through the season was lower than SWP measured for healthy grapevines. For both seasons, there were significant differences in SWP measurements between healthy and GFLV-infected grapevines of ‘Schioppettino’ trained using the single Guyot training system.
SWP and RHC of the GFLV-infected grapevines were reduced compared to the healthy controls. The water deficit triggered the production of ABA, which induced the expression of the stress-related gene RD22. Additionally, this study shows that the WRKY gene that is involved in the ABA signalling network is regulated by water deficit. Plant defence responses to water stress also included up-regulation of the F3H2 and LDOX genes, which are involved in anthocyanins synthesis. GFLV infection significant impacted upon the expression of genes involves in ABA biosynthesis, as NCED1 and NCED2, and upon two genes involved in the early stages of anthocyanins synthesis, as CHS2 and F3H1. We also showed that the combination of grapevine cultivar, training system, and environmental conditions impacts on gene expression Found in: ključnih besedah Summary of found: ...reduced compared to the healthy controls. The water deficit triggered the production of ABA, which... Keywords: Vitis vinifera L., grapevine, Grapevine fanleaf virus, GFLV, grapevine disease, virus titre, distribution, fluctuation, ELISA, qPCR, ABA, drought, water status, water deficit, SWP, RHC, anthocyanins, gene expression Published: 27.07.2015; Views: 7372; Downloads: 407
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6. Measurement of the water-Cherenkov detector response to inclined muons using an RPC hodoscopeMarko Zavrtanik, Danilo Zavrtanik, Lili Yang, Serguei Vorobiov, Darko Veberič, Marta Trini, Samo Stanič, Ahmed Saleh, Gašper Kukec Mezek, Andrej Filipčič, Pedro Assis, 2015, published scientific conference contribution Abstract: The Pierre Auger Observatory operates a hybrid detector
composed of a Fluorescence Detector and a Surface Detector
array. Water-Cherenkov detectors (WCD) are the building blocks
of the array and as such play a key role in the detection of
secondary particles at the ground. A good knowledge of the
detector response is of paramount importance to lower
systematic uncertainties and thus to increase the capability
of the experiment in determining the muon content of the
extensive air showers with a higher precision.
In this work we report on a detailed study of the detector
response to single muons as a function of their trajectories
in the WCD. A dedicated Resistive Plate Chambers (RPC)
hodoscope was built and installed around one of the detectors.
The hodoscope is formed by two stand-alone low gas flux
segmented RPC detectors with the test water-Cherenkov detector
placed in between. The segmentation of the RPC detectors is of
the order of 10 cm. The hodoscope is used to trigger and
select single muon events in different geometries. The signal
recorded in the water-Cherenkov detector and performance
estimators were studied as a function of the trajectories of
the muons and compared with a dedicated simulation.
An agreement at the percent level was found, showing that the
simulation correctly describes the tank response. Found in: ključnih besedah Keywords: Pierre Auger Observatory, Water-Cherenkov detectors, detector calibration, inclined cosmic ray muons, Resistive Plate Chambers (RPC) hodoscope Published: 03.03.2016; Views: 4013; Downloads: 192
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8. Regenerated cellulose films combined with glycerol and polyvinyl alcohol: Effect of moisture content on the physical propertiesManuel Vazquez, Gonzalo Velazquez, Patricia Cazón, 2020, original scientific article Abstract: Regenerated cellulose-based films combined with glycerol and polyvinyl alcohol (PVOH) show interesting UV- light barrier properties, with potential application in food packaging to prevent oxidative deterioration. How- ever, these materials are sensitive to moisture, and their properties could be modified as a function of the relative humidity. Hence, the objective of the present work was to evaluate the changes in the main properties of re- generated cellulose-glycerol-PVOH films depending on the relative humidity. Using the GAB adsorption iso- therms, the moisture content was related with the water activity of the films at several relative humidity conditions. According to the obtained results, water molecules manifested a plasticizing effect modifying the mechanical, water vapour permeability and optical properties of the developed films. Tensile strength and Young’s modulus values ranged from 92.65 to 17.57 MPa and from 3639.09 to 227.89 MPa, respectively. Both of them decreased when the moisture content increased. The mechanical resistance to deformation of films enhanced at high moisture content, changing from 5.88 to 15.97% and from 0.59 to 2.97 mm in the tensile and puncture test, respectively. This effect was also observed for the burst strength. Water vapour permeability increased from 5.15⋅10?10 to 5.44⋅10?9 g/ms Pa when the moisture content increased, being more significative at higher values. No significant variations were observed in the UV-VIS transmittance at different moisture contents. The obtained results allow expanding the knowledge of the behavior of films based on regenerated cellulose. Found in: ključnih besedah Summary of found: ...the moisture content was related with the water activity of the films at several relative... Keywords: Adsorption isotherms, Plasticization, Regenerated cellulose, Water vapour permeability, Moisture content Published: 09.12.2020; Views: 1769; Downloads: 0
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10. Highly Sensitive Determination of Pyoverdine in Cloud Water by HPLC-Thermal Lens SpectrometryLeja Goljat, Mitja Martelanc, Virginie Vinatier, Anne-Marie Delort, Mladen Franko, 2016, published scientific conference contribution abstract Abstract: New method for pyoverdine and Fe(III)-pyoverdine detection was developed. Two isomers of pyoverdine and two isomers of Fe(III)-pyoverdine were separated isocraticaly on reversed-phase (RP)-C18 chromatograhic column and detected by DAD, FLD and TLS. HPLC-TLS method enables separation and determination of pyoverdine and Fe(III)-pyoverdine in a single run and excels in superior sensitivities when compared to conventional HPLC-DAD system. Found in: ključnih besedah Keywords: Pyoverdine, Fe(III)-pyoverdine, cloud water, high-performance liquid chromatography, thermal lens spectrometry Published: 04.07.2016; Views: 4747; Downloads: 0 |