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Computational design of customised nanobodies for biotechnological applications
Sara Fortuna, Ario de Marco, Miguel Soler, 2016, unpublished conference contribution

Abstract: In silico modeling to improve the biophysical characteristics of recombinant single-domain antibodies
Found in: osebi
Keywords: nanobodies, modeling, protein stability, antibody humanization, molecular dynamics
Published: 26.04.2016; Views: 3946; Downloads: 0
.pdf Fulltext (2,42 MB)

Lo sviluppo biotecnologico della pallottola magica proposta nel 1906 da Paul Ehrlich, padre della chemioterapia
Ario de Marco, invited lecture at foreign university

Abstract: Description of Paul Ehrlich contribution to modern medicine
Found in: osebi
Keywords: targeted therapy, antibodies, biomarkers, molecule functionalization, differential staining
Published: 26.04.2016; Views: 4027; Downloads: 0
.pdf Fulltext (4,05 MB)

Selection criteria and engineering of nanobodies for oncological applications
Ario de Marco, invited lecture at foreign university

Abstract: Lecture relative to the biotechnological features of nanobodies
Found in: osebi
Keywords: nanobodies, phage display, antibody engineering, panning
Published: 14.07.2016; Views: 3705; Downloads: 0
.pdf Fulltext (22,12 KB)

NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Jean-Christophe Rain, Laetitia Ligat, Ario de Marco, Emilie Lemesre, Laura Keller, Virginie Bernard, Nicolas Bery, Sandrine Moutel, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, original scientific article

Abstract: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Found in: osebi
Keywords: nanobodies, synthetic phage display library, in vitro panning
Published: 17.08.2016; Views: 4259; Downloads: 238
.pdf Fulltext (4,32 MB)

A Nano-Imunoassay based on fluorescence and atomic force spectroscopy for the detection of circulating cancer biomarkers: The case of HER2 positive breast cancer
Ario de Marco, Alessandro Bosco, Pietro Parisse, Elena Ambrosetti, Loredana Casalis, Elsa Tagliabue, 2016, published scientific conference contribution abstract

Abstract: A promising new approach for the detection of biomarkers in patient sera
Found in: osebi
Keywords: AFM, diagnostic antibodies, HER2
Published: 17.08.2016; Views: 3769; Downloads: 0
.pdf Fulltext (854,47 KB)
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Molecular dynamics simulations and docking enable to explore the biophysical factors controlling the yields of engineered nanobodies
Ario de Marco, Miguel Soler, Sara Fortuna, 2016, original scientific article

Abstract: Nanobodies (VHHs) have proved to be valuable substitutes of conventional antibodies for molecular recognition. Their small size represents a precious advantage for rational mutagenesis based on modelling. Here we address the problem of predicting how Camelidae nanobody sequences can tolerate mutations by developing a simulation protocol based on all-atom molecular dynamics and wholemolecule docking. The method was tested on two sets of nanobodies characterized experimentally for their biophysical features. One set contained point mutations introduced to humanize a wild type sequence, in the second the CDRs were swapped between single-domain frameworks with Camelidae and human hallmarks. The method resulted in accurate scoring approaches to predict experimental yields and enabled to identify the structural modifications induced by mutations. This work is a promising tool for the in silico development of single-domain antibodies and opens the opportunity to customize single functional domains of larger macromolecules
Found in: osebi
Keywords: nanobodies, molecular dynamics, modeling, antibody solubility
Published: 11.10.2016; Views: 4023; Downloads: 240
.pdf Fulltext (1,95 MB)

Nanobodies for research & clinical applications
Ario de Marco, invited lecture at foreign university

Abstract: Nanobody technology evolved rapidly in the last years and now is available for applications in basic sciences as well as in diagnostics and therapy
Found in: osebi
Keywords: nanobodies, antibody functionalization, phage display panning, recombinant antibody production
Published: 11.10.2016; Views: 4246; Downloads: 0
.pdf Fulltext (773,47 KB)

Nanobody technology: principles & applications
Ario de Marco, unpublished invited conference lecture

Found in: osebi
Keywords: nanobodies, nanotechnologies, biomarker identification
Published: 29.03.2017; Views: 3788; Downloads: 0
.pdf Fulltext (114,69 KB)

Assessment of the actual antibody-antigen binding conditions at the surface of biological membranes
Ario de Marco, 2016, published scientific conference contribution abstract (invited lecture)

Found in: osebi
Keywords: Exosomes, avidity effect, AFM
Published: 30.11.2016; Views: 4107; Downloads: 0
.pdf Fulltext (211,28 KB)
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