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1.
Early stress detection in forest trees using a nanobody-functionalized electrochemical biosensor for ascorbate peroxidase
Claudia D'Ercole, Rossella Svigelj, Tanja Mrak, Ario De Marco, 2025, original scientific article

Abstract: Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature such as heavy metal contamination, drought, or pest infestations which may lead to their massive decline. We designed a comprehensive approach for isolating, producing and functionalizing reagents suitable for the affordable detection of forest plant stress biomarkers with the aim to provide quantitative data to assess plant stress fluctuation and, possibly, to design mitigation strategies. We first optimized a panning protocol to recover nanobodies targeting shared sequences that could cross-react with both Pisum sativum and Populus nigra ascorbate peroxidase (APX). After their production as recombinant constructs and their extensive biophysical and biochemical characterization, such reagents were exploited as the immunocapture element of an electrochemical biosensor conceived as a potential point-of-care device. Such biosensor could detect both pea and poplar APX in leaf extracts and could be used to clearly discriminate between control and heavy metal-stressed poplar plants based on their APX activity, even before the appearance of any phenotypic symptom. The combination of fast and inexpensive reagent production with the development of portable diagnostics opens the opportunity for large-scale, on-site surveys of forest trees.
Keywords: plant stress, scavengers, diagnostics, nanobodies, biosensors
Published in RUNG: 11.04.2025; Views: 227; Downloads: 2
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Common targetable biomarkers in canine hemangiosarcoma and human angiosarcoma
Huyen Thuc Tran Luong, Dominique Revets, Matteo De March, Sofie Vercammen, Hilde De Rooster, Ario De Marco, Antonio Cosma, 2024, published scientific conference contribution abstract

Keywords: comparative oncology, angiosarcoma, hemangiosarcoma, nanobodies
Published in RUNG: 18.03.2025; Views: 336; Downloads: 0
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Recent advances in recombinant production of soluble proteins in E. coli
Ario De Marco, 2025, review article

Keywords: recombinant proteins, E. coli, protein yield, protein functionality, protein solubility
Published in RUNG: 16.01.2025; Views: 584; Downloads: 3
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Scienza senza confini : l'ǂesempio dei profitaliani nell'ateneo di Nova Gorica
2024, interview

Published in RUNG: 05.12.2024; Views: 820; Downloads: 4
URL Link to file

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Harnessing in vitro selection opportunities for obtaining epitope-specific binders : lecture at the CDKL5 Forum 2024, Cambridge, MA, USA, 27 October 2024
Ario De Marco, 2024, unpublished conference contribution

Keywords: CDKL5, nanobodies, biopanning
Published in RUNG: 05.11.2024; Views: 803; Downloads: 0
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Recombinant antibodies
Ario De Marco, 2024, independent scientific component part or a chapter in a monograph

Keywords: antibody fragments, nanobodies, recombinant expression
Published in RUNG: 20.09.2024; Views: 1135; Downloads: 2
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