1. Early stress detection in forest trees using a nanobody-functionalized electrochemical biosensor for ascorbate peroxidaseClaudia D'Ercole, Rossella Svigelj, Tanja Mrak, Ario De Marco, 2025, original scientific article Abstract: Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature such as heavy metal contamination, drought, or pest infestations which may lead to their massive decline. We designed a comprehensive approach for isolating, producing and functionalizing reagents suitable for the affordable detection of forest plant stress biomarkers with the aim to provide quantitative data to assess plant stress fluctuation and, possibly, to design mitigation strategies. We first optimized a panning protocol to recover nanobodies targeting shared sequences that could cross-react with both Pisum sativum and Populus nigra ascorbate peroxidase (APX). After their production as recombinant constructs and their extensive biophysical and biochemical characterization, such reagents were exploited as the immunocapture element of an electrochemical biosensor conceived as a potential point-of-care device. Such biosensor could detect both pea and poplar APX in leaf extracts and could be used to clearly discriminate between control and heavy metal-stressed poplar plants based on their APX activity, even before the appearance of any phenotypic symptom. The combination of fast and inexpensive reagent production with the development of portable diagnostics opens the opportunity for large-scale, on-site surveys of forest trees.
Keywords: plant stress, scavengers, diagnostics, nanobodies, biosensors
Published in RUNG: 11.04.2025; Views: 227; Downloads: 2
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2. Metagenomics studies in aquaculture systems : big data analysis, bioinformatics, machine learning and quantum computingOrkid Coskuner-Weber, Semih Alpsoy, Ozgur Yolcu, Egehan Teber, Ario De Marco, Spase Shumka, 2025, review article Keywords: marine biology, machine learning, diagnostics
Published in RUNG: 07.04.2025; Views: 267; Downloads: 0
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3. Understanding the nanoscale organization of natural killer cell receptor NKp30 at the singlemolecule levelBarbora Kalouskova, Ondřej Skořepa, Jan Bláha, Lucia Cikatricisová, Ario De Marco, Ondřej Vaněk, Mario Brameshuber, 2024, published scientific conference contribution abstract Keywords: advanced microscopy, nanobodies, fluorescent proteins
Published in RUNG: 24.03.2025; Views: 296; Downloads: 0
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4. Common targetable biomarkers in canine hemangiosarcoma and human angiosarcomaHuyen Thuc Tran Luong, Dominique Revets, Matteo De March, Sofie Vercammen, Hilde De Rooster, Ario De Marco, Antonio Cosma, 2024, published scientific conference contribution abstract Keywords: comparative oncology, angiosarcoma, hemangiosarcoma, nanobodies
Published in RUNG: 18.03.2025; Views: 336; Downloads: 0
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5. Development of molecular methods for the comprehensive evaluation of stress conditions affecting forest trees : dissertationClaudia D'Ercole, 2025, doctoral dissertation Keywords: protein complexes, adhirons, nanobodies, plant stress, immunoreagents, protoplasts, ascorbate peroxidase, dissertations
Published in RUNG: 04.02.2025; Views: 668; Downloads: 9
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8. Computational study of the HLTF ATPase remodeling domain suggests its activity on dsDNA and implications in damage toleranceMartin Ljubič, Claudia D'Ercole, Yossma Waheed, Ario De Marco, Jure Borišek, Matteo De March, 2024, original scientific article Keywords: DNA repairing mechanism, protein modeling, chromatin remodeler, helicase-like transcription factor
Published in RUNG: 15.11.2024; Views: 1120; Downloads: 2
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