11. Quality control of protein reagents for the improvement of research data reproducibilityArio De Marco, Nick Berrow, Mario Lebendiker, Maria Garcia-Alai, Stefan H. Knauer, Blanca Lopez-Mendez, Andre Matagne, Annabel Parret, Kim Remans, Stephan Uebel, Bertrand Raynal, 2021, short scientific article Keywords: protein quality, protein aggregation, data reproducibility
Published in RUNG: 17.05.2021; Views: 2090; Downloads: 13
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12. Intercomparison and characterization of 23 Aethalometers under laboratory and ambient air conditions: : procedures and unit-to-unit variabilitiesAndrea Cuesta-Mosquera, Griša Močnik, Luka Drinovec, Thomas Müller, Sascha Pfeifer, Maria Cruz Minguillon, Björn Briel, Paul Buckley, Vadimas Dudoitis, Javier Fernández-García, 2021, original scientific article Abstract: Aerosolized black carbon is monitored worldwide to quantify its impact on air quality and climate. Given its importance, measurements of black carbon mass concentrations must be conducted with instruments operating in quality-checked and ensured conditions to generate data which are reliable and comparable temporally and geographically.
In this study, we report the results from the largest characterization and intercomparison of filter-based absorption photometers, the Aethalometer model AE33, belonging to several European monitoring networks. Under controlled laboratory conditions, a total of 23 instruments measured mass concentrations of black carbon from three well-characterized aerosol sources: synthetic soot, nigrosin particles, and ambient air from the urban background of Leipzig, Germany. The objective was to investigate the individual performance of the instruments and their comparability; we analyzed the response of the instruments to the different aerosol sources and the impact caused by the use of obsolete filter materials and the application of maintenance activities.
Differences in the instrument-to-instrument variabilities from equivalent black carbon (eBC) concentrations reported at 880 nm were determined before maintenance activities (for soot measurements, average deviation from total least square regression was −2.0 % and the range −16 % to 7 %; for nigrosin measurements, average deviation was 0.4 % and the range −15 % to 17 %), and after they were carried out (for soot measurements, average deviation was −1.0 % and the range −14 % to 8 %; for nigrosin measurements, the average deviation was 0.5 % and the range −12 % to 15 %). The deviations are in most of the cases explained by the type of filter material employed by the instruments, the total particle load on the filter, and the flow calibration.
The results of this intercomparison activity show that relatively small unit-to-unit variability of AE33-based particle light absorbing measurements is possible with well-maintained instruments. It is crucial to follow the guidelines for maintenance activities and the use of the proper filter tape in the AE33 to ensure high quality and comparable black carbon (BC) measurements among international observational networks.
Keywords: black carbon, aerosol, absorption, filter absorption photometer, aethaloemter
Published in RUNG: 03.05.2021; Views: 2032; Downloads: 0
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13. Quality control of purified proteins to improve data quality and reproducibility: results from a large-scale surveyNick Berrow, Ario De Marco, Mario Lebendiker, Maria Garcia-Alai, Stefan H. Knauer, Blanca Lopez-Mendez, Andre Matagne, Annabel Parret, Kim Remans, Stephan Uebel, Bertrand Raynal, 2021, original scientific article Keywords: protein quality, biophysical analysis, protein aggregates
Published in RUNG: 22.04.2021; Views: 2115; Downloads: 0
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15. Taming the topology of calix[4]arene-based 2D-covalent organic frameworks : interpenetrated vs noninterpenetrated frameworks and their selective removal of cationic dyesBikash Garai, Dinesh Shetty, Tina Škorjanc, Felipe Gándara, Nawavi Naleem, Sabu Varghese, Sudhir Kumar Sharma, Maria Baias, Ramesh Jagannathan, Mark Anthony Olson, 2021, original scientific article Abstract: A bowl-shaped calix[4]arene with its exciting host–guest chemistry is a versatile supramolecular building block for the synthesis of distinct coordination cages or metal–organic frameworks. However, its utility in the synthesis of crystalline covalent organic frameworks (COFs) remains challenging, presumably due to its conformational flexibility. Here, we report the synthesis of a periodic 2D extended organic network of calix[4]arenes joined by a linear benzidine linker via dynamic imine bonds. By tuning the interaction among neighboring calixarene units through varying the concentration in the reaction mixture, we show the selective formation of interpenetrated (CX4-BD-1) and non-interpenetrated (CX4-BD-2) frameworks. The cone-shaped calixarene moiety in the structural backbone allows for the interweaving of two neighboring layers in CX4-BD-1, making it a unique example of interpenetrated 2D layers. Due to the high negative surface charge from calixarene units, both COFs have shown high performance in charge-selective dye removal and an exceptional selectivity for cationic dyes irrespective of their molecular size. The charge distribution of the COFs and the resulting selectivity for the cationic dyes were further investigated using computational methods.
Keywords: dyes and pigments, covalent organic frameworks, adsorption, layers, chemical structure
Published in RUNG: 16.03.2021; Views: 2230; Downloads: 0
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16. Self-assembly and regulation of protein cages frompre-organised coiled-coil modulesFabio Lapenta, Jana Aupič, Marco Vezzoli, Žiga Strmšek, Stefano Da Vela, Dmitrii Ivanovich Svergun, Jose Maria Carazo, Roberto Melero, Roman Jerala, 2021, original scientific article Published in RUNG: 11.02.2021; Views: 2125; Downloads: 116
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17. Action Learning Sets - EmindS Training MaterialsPetroula Mavrkiou, Peter Purg, Kirsi Maasalo, Dimitrios Doukas, Haris Tsitouras, Maria Koutiva, Christiana Knais, reviewed university, higher education or higher vocational education textbook Keywords: action learning, materials, entrepreneurship, interdisciplinary, entrecomp
Published in RUNG: 05.01.2021; Views: 2228; Downloads: 0
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18. Installation and Replication Manual - EmindS projectPetroula Mavrikiou, Peter Purg, Kirsi Maasalo, Dimitrios Doukas, Haris Tsitouras, Maria Koutiva, Christiana Knais, other educational material Keywords: manual, entrepreneurship, interdisciplinary, entrecomp
Published in RUNG: 05.01.2021; Views: 2260; Downloads: 0
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19. Assessment tool for the entrepreneurship competence - EmindS projectPeter Purg, Petroula Mavrikiou, Kirsi Maasalo, Dimitrios Doukas, Haris Tsitouras, Maria Koutiva, Christiana Knais, other educational material Keywords: assessment, entrepreneurship, interdisciplinary, entrecomp
Published in RUNG: 05.01.2021; Views: 2196; Downloads: 0
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20. Key competences and respective learning outcomes for the Entrepreneurial Mindset in Europe - EminDS projectPetroula Mavrikiou, Peter Purg, Kirsi Maasalo, Dimitrios Doukas, Haris Tsitouras, Maria Koutiva, Christiana Knais, final research report Keywords: outcomes, entrepreneurship, interdisciplinary, entrecomp
Published in RUNG: 05.01.2021; Views: 2288; Downloads: 0
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