41. Regulation and controlling the motility properties of Pseudomonas aeruginosaFazlurrahman Khan, Dung Thuy Nguyen Pham, Sandra Oloketuyi, Young-Mog Kim, 2020, review article Abstract: Chronic infections caused by Pseudomonas aeruginosa have been a major concern as their spread and mortality continue to be on the rise. These infections are majorly attributed to biofilm formation via sequential steps where motility plays an essential role in initial attachment of bacterial cells onto biotic and abiotic surfaces, thereby contributing to multi-drug resistance among pathogens. Therefore, attenuating motility properties can be considered as highly potential for controlling P. aeruginosa biofilm formation. This strategy has employed the use of various natural and chemically synthesized compounds. The present review article explained the importance and regulation of different types of motilities properties. Furthermore, it also covered several important alternative approaches using anti-motility agents which could be helpful for controlling P. aeruginosa biofilm-associated infections. Further studies are required for in-depth understandings about the mechanisms of motilities controlling of these molecules at molecular levels.
Keywords: Biofilm, Motility, Attenuation, Antibiofilm drugs, Pseudomonas aeruginosa
Published in RUNG: 12.01.2021; Views: 2647; Downloads: 0
This document has many files! More... |
42. Spremljanje, ugotavljanje in zagotavljanje kakovosti: poročilo za študijsko leto 2019/2020 : Univerza v Novi Gorici. : poročilo za študijsko leto 2019/2020 : Univerza v Novi Gorici.Iztok Arčon, Jana Beguš, Blaž Belec, Martina Bergant Marušič, Lorena Butinar, IMRE CIKAJLO, Saša Dobričič, Sandra Gardonio, Renata Kop, Martin Knez, Boštjan Potokar, Branka Mozetič Vodopivec, Egon Pavlica, Samo Stanič, Tea Stibilj Nemec, Željko Oset, VERONIA Piccinini, Peter Purg, Arthur Stepanov, Aljaž Rener, Nives Štefančič, Ana Toroš, Tanja Urbančič, Vanesa Valentinčič, NIKA VODOPIVEC, treatise, preliminary study, study Keywords: samoevalvacijsko poročilo
Published in RUNG: 23.12.2020; Views: 3359; Downloads: 0
This document has many files! More... |
43. Ga2Se3 Nanowires via Au-Assisted Heterovalent Exchange Reaction on GaAsFederico Berto, Niloofar Haghighian, Katja Ferfolja, Sandra Gardonio, Mattia Fanetti, Faustino Martelli, Valentina Mussi, Vladimir G. Dubrovskii, Igor V. Shtrom, Alfonso Franciosi, Silvia Rubini, 2020, original scientific article Keywords: nanowires, Gallium arsenide, Gallium Selenide, gold, molecular beam epitaxy
Published in RUNG: 15.12.2020; Views: 2437; Downloads: 0
This document has many files! More... |
44. |
45. Fast and efficient removal of paraquat in water by porous polycalix [n] arenes (n= 4, 6, and 8)Dinesh Shetty, Sandra Boutros, Tina Škorjanc, Bikash Garai, Zouhair Asfari, Jesus Raya, Ali Trabolsi, 2020, original scientific article Keywords: paraquat, adsorption, calixarene, covalent polymers, water purification
Published in RUNG: 02.09.2020; Views: 2447; Downloads: 0
This document has many files! More... |
46. |
47. |
48. FUNCTIONALIZED NANOBODIES AS DIAGNOSTIC REAGENTS FOR THE DETECTION OF TOXIC MICROALGAESandra Oloketuyi, 2020, doctoral dissertation Abstract: Early detection and monitoring of toxic microalgae remains a challenge that will be necessary to overcome for understanding harmful algal bloom events and their consequent ecological and environmental impacts. Although there are diagnostic techniques based on microscopy, fluorescence in situ hybridization (FISH), DNA microarrays, real-time PCR, sandwich hybridization assays employed for the detection of harmful algal species, such techniques are not suitable for field monitoring and especially for the quantification of Alexandrium minutum due to abundance of cryptic species that cannot be differentiated based on morphology or genomic traits. These approaches are also time-consuming and laborious, hence there is need for alternative simple, rapid and cost-effective methods. In this study, our aim was to develop different nanobody-based capture strategies for the detection of A. minutum. Nanobodies were successfully displayed on the surface of Escherichia coli to facilitate detection of A. minutum. The method is simple and cost effective as no antibody purification step is necessary. As an alternative, A. minutum was quantified by exploiting the peroxidase activity of a G-quadruplex DNAzyme covalently bound to nanobodies and by means of a label free electrochemical immunosensor which exploit nanobodies bound via an irreversible SpyTag-SpyCatcher system as the specific immunocapture element. Such immunosensor was prepared by immobilizing the SpyTagged anti-A. minutum nanobody (SpyTagged C1) by means of a selfassembled monolayer (SAMs) of L- cysteine (L-Cys) displayed onto a gold nanoparticle-modified glassy carbon electrode. Electrochemical Impedance Spectroscopy (EIS) was performed to measure the electrical response of the circuit as the function of A. minutum immunocaptured cells. The results of EIS studies confirmed that the sensing chip detected A. minutum selectively, exhibited a wide dynamic range spanning from 103 to 109 cell L-1 and a limit of detection of 3.1× 103 cell L-1. The immunosensor data can be recorded and then analyzed with a portable potentiostat. Such device can be interfaced with a smart phone, a condition suitable for the rapid A. minutum quantification in situ.
Keywords: Alexandrium minutum, nanobodies, bioreagents, immunosensor, toxic microalgae
Published in RUNG: 17.06.2020; Views: 4066; Downloads: 232
 Full text (2,95 MB) |
49. Chemical Instability of an Interface between Silver and Bi2Se3 Topological Insulator at Room TemperatureKatja Ferfolja, Matjaž Valant, Iuliia Mikulska, Sandra Gardonio, Mattia Fanetti, 2018, original scientific article Abstract: Understanding an interaction at an interface between a topological insulator and a metal is of critical importance when designing electronic and spintronic devices or when such systems are used in catalysis. In this paper, we report on a chemical instability of the interface between Bi2Se3 and Ag studied by X-ray powder diffraction and electron microscopy. We present strong experimental evidence of a redox solid-state reaction occurring at the interface with kinetics that is significant already at room temperature. The reaction yields Ag2Se, AgBiSe2, and Bi. The unexpected room-temperature chemical instability of the interface should be considered for all future theoretical and applicative studies involving the interface between Bi2Se3 and Ag.
Keywords: topological insulators, Ag, thin metal films, interfaces, redox reaction
Published in RUNG: 17.06.2020; Views: 2912; Downloads: 0
This document has many files! More... |
50. |
