1. High spatial heterogeneity of water stress levels in Refošk grapevines cultivated in Classical KarstFrancesco Petruzzellis, Sara Natale, Luca Bariviera, Alberto Calderan, Alenka Mihelčič, Jan Reščič, Paolo Sivilotti, Katja Šuklje, Klemen Lisjak, Andreja Vanzo, Andrea Nardini, 2022, original scientific article Keywords: Classical Karst, water status, drought stress, grape quality, phenolic profile, vitis vinifera Published in RUNG: 11.11.2021; Views: 3128; Downloads: 0 This document has many files! More... |
2. Facilitating green care improvements in viticultureMelita Sternad Lemut, Lorena Butinar, Adesida Rowland, Urban Česnik, Tjaša Jug, Paolo Sivilotti, Urška Vrhovšek, 2019, published scientific conference contribution Abstract: The growing demand of consumers for environment-friendly and safe food products already forced many winemakers to restrict the application of synthetic fungicides to earlier season, trying to reduce the risk of residues being detected in wines. Main risk for infection by B. cinerea, however, occurs later in the season. To support an improvement in whole-season B. cinerea green care, the potential of yeasts as biocontrol agent (PBA) was studied. Selected yeasts were tested for their antifungal activities. Furthermore, a field experiment in Pinot noir and Pinot gris vineyards was designed to study different combinations of optimized canopy microclimate manipulation (CMM) techniques and PBA application. In experimental conditions, PBA's ability to maintain appropriate population density for disease prevention was observed. Grape and wine quality parameters were evaluated. Analytical approach of metabolomics was applied to upgrade the understanding of potentially induced changes in plant metabolism.
Based on yeasts testing results, biocontrol yeast P. guilliermondii ZIM 624 was selected and after its field application, we were able to detect its suitable density on grapes until harvest. In case of early defoliation for both varieties smaller grape compactness was confirmed together with lower yield. Among grape basic quality parameters, optimized techniques showed positive effect on sugar content, but unexpectedly, in the treatments with biocontrol yeasts the trends toward higher acidity were noticed in Pinot gris. CMM and PBA introduction indicated some effects on grapevine biosynthetic behaviour but the differences in content of these metabolites compared to control were poorly detected in final wines with exception of mainly some flavonols, stilbenes and flavan-3-ols (and consequently total phenolic content). Keywords: sustainable viticulture, Botrytis cinerea, yeasts, biocontrol, canopy microclimate manipulation, metabolomics, grapevine metabolites Published in RUNG: 28.02.2020; Views: 4872; Downloads: 0 This document has many files! More... |
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4. VPLIV ODTRANJEVANJA LISTOV IN UPORABE MREŽ ZA ZAŠČITO PRED PTIČI NA KOLIČINO PRIDELKA TER KAKOVOSTNE PARAMETRE GROZDJA IN VINA SORTE SAUVIGNONASSE (VITIS VINIFERA L.)Aljaž Namar, 2018, undergraduate thesis Abstract: Odstranjevanje listov v območju grozdov in uporaba mrež za zaščito pred ptiči sta pogosto uporabljeni vinogradniški strategiji, ki vplivata na mikroklimatske pogoje v grmu vinske trte ter posledično na količinske in kakovostne parametre pridelka. V okviru diplomskega dela smo v vinogradu sorte 'Sauvignonasse' v Pristavem (Brda), po načelu naključne razporeditve blokov trt, zastavili primerjavo med petimi ukrepi: UNT (brez tretiranja), ELR (razlistanje pred cvetenjem), LLR (razlistanje po cvetenju), REN (razlistanje po cvetenju in senčenje z rdečimi mrežami) ter BKN (razlistanje po cvetenju in senčenje s črnimi mrežami). Tekom dozorevanja smo v odvisnosti od ukrepa spremljali osnovne kakovostne, ob trgatvi pa tudi količinske parametre pridelka. Grozdje smo ločeno vinificirali in v vinih določili osnovne kakovostne parametre, vsebnost hlapnih tiolov ter opravili senzorično analizo. Z obravnavanimi ukrepi smo le v manjši meri vplivali na kakovostne parametre grozdja, medtem, ko smo, predvsem z ukrepom ELR, pomembneje vplivali na količino pridelka in težo grozdov. V vinih smo (v korist obravnavanjem ELR, LLR in REN) opazili razlike v koncentracijah sladkorja prostega ekstrakta, poleg različnih trendov vsebnosti skupnih titracijskih kislin pa smo z ukrepi povzročili tudi nekaj razlik v vsebnostih posameznih kislin (vinska, jabolčna) ter skupnih hlapnih kislin. V primerjavi s kontrolo smo opazili povečanje vsebnosti 4MMP pri obravnavnjih BKN in ELR ter povečanje 3MH pri LLR, BKN in REN. Natančnejši pregled povzročenih sprememb in rezultatov (enoletnih) primerjav je nakazal, da učinki na opazovane parametre niso tako preprosti in linearni, da bi omogočili sistematično optimizacijo oz. da so medsebojni vplivi različnih kombinacij vinogradniških strategij na parametre grozdja kompleksni ter jih moramo uvajati nadvse previdno. Keywords: 'Sauvignonasse' (Vitis vinifera L.), odstranjevanje listov v območju grozdov, mreže za zaščito pred priči, mikroklima, osnovni količinski in kakovostni parametri grozdja in vina, hlapni tioli. Published in RUNG: 06.03.2018; Views: 7495; Downloads: 233 Full text (1,12 MB) |
5. Study of grapevine early leaf removal sustainability potential by means of targeted metabolomic approachMelita Sternad Lemut, Paolo Sivilotti, Lorena Butinar, Ajda Lemut, Urška Vrhovšek, 2017, published scientific conference contribution abstract Keywords: Pinot gris, early leaf removal, sustainable viticulture, grape compactness, targeted metabolomics, phenolic compounds Published in RUNG: 21.09.2017; Views: 6005; Downloads: 0 This document has many files! More... |
6. Combined effects of early season leaf removal and climatic conditions on aroma precursors in ‘Sauvignon blanc’ grapesPaolo Sivilotti, Rachele FALCHI, Jose Carlos Herrera, Branka Škvarč, Lorena Butinar, Melita Sternad Lemut, Marijan Bubola, Paolo SABBATINI, Klemen Lisjak, Andreja Vanzo, 2017, original scientific article Abstract: Early leaf removal around the cluster zone is a common technique applied in cool climate viticulture, to regulate yield components and improve fruit quality. Despite the increasing amount of information on early leaf removal and its impact on total soluble solids, anthocyanins and polyphenols, less is known regarding aroma compounds. In order to verify the hypothesis that defoliation, applied before or after flowering, could impact the biosynthesis of thiol precursors, we performed a two year (2013 and 2014) experiment on Sauvignon blanc. We provided evidence that differential accumulation of thiols precursors in berries is affected by the timing of defoliation and this impact was related to modifications in biosynthetic pathway. Furthermore, the possible interaction between leaf removal treatment and seasonal weather conditions, and its effect on the biosynthesis of volatile precursors are discussed. Our results suggested that in Sauvignon blanc the relative proportion of 4-S-glutathionyl-4-methylpentan-2-one (G-4MSP) and 3-S-glutathionylhexan-1-ol (G-3SH) precursors can be affected by defoliation, and this could be related to the induction of two specific genes encoding glutathione-S-transferases (VvGST3 and VvGST5), while no significant effects on basic fruit chemical parameters, polyphenols and methoxypyrazines were ascertained under our experimental conditions. Keywords: early leaf removal, cluster exposure, thiol precursors, methoxypyrazines, glutathione S-transferase, Vitis vinifera Published in RUNG: 31.08.2017; Views: 5169; Downloads: 0 This document has many files! More... |
7. Alternativne vinogradniške in vinarske tehnologije: kakovostni, trajnostni in senzorični potencial : /Melita Sternad Lemut, Paolo Sivilotti, Lorena Butinar, Jana Laganis, Urška Vrhovšek, 2017, published scientific conference contribution abstract (invited lecture) Keywords: odstajevanju listov v predelu grozdov, trajnostno vinogradništvo, Botrytis cinerea, mikrobna ekologija vinske trte, alternativne vinarske tehnologije, senzorika vina. Published in RUNG: 29.06.2017; Views: 6134; Downloads: 258 Full text (15,95 KB) |
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9. EFFECT OF TIMING OF LEAF REMOVAL ON YIELD PARAMETERS, GRAPE AND WINE QUALITY OF VITIS VINIFERA L. CV. 'SAUVIGNON BLANC' Daniela MARKOVICDaniela Markovic, 2016, undergraduate thesis Abstract: Z odstranjevanjem listov v predelu grozdov se lahko spremeni mikroklima grozdov, ki lahko izzove spremembe v sekundarnem metabolizmu vinske trte, ki so odvisne tudi od časa izvedbe ukrepa. Uravnavanje sinteze aromatskih komponent je zanimivo pri belih sortah kot je sorta 'Sauvignon blanc', kjer so metoksipirazini in tioli tisti, ki oblikujejo značilno aromo vina te sorte.
Eksperiment diplomske naloge smo zastavili na Oslavju (Gorica, Italija) v vinogradu sorte grozdja 'Sauvignon blanc' (Vitis vinifera L.). Želeli smo preveriti vpliva odstranjevanja listov v predelu grozdja pred in po cvetenju na količino in kakovost pridelka ter aromatske lastnosti grozdja in vina.
Količinski parametri pridelka so pokazali majhne vplive izvedenih ukrepov razlistanja; pozno razlistanje na količino pridelka ni vplivalo, v nasprotju z razlistanjem pred cvetenjem, kjer smo opazili rahlo zmanjšanje pridelka po trsu in mase grozdov. Tudi pri osnovnih zrelostnih parametrih smo opazili manjše spremembe, predvsem večje vsebnosti topne suhe snovi pri razlistanju pred cvetenjem.
Pri aromatskih komponentah smo zaznali vpliv razlistanja pred cvetenjem na količino prekurzorjev tiolov tako v grozdju kot tudi vinu; večjo vsebnost 4-merkapto-4-metilpentan-2-on (4MMP) in nižje koncentracije 3-merkaptoheksan-1-ol (3MH). S poznejšim odstranjevanjem listov smo zmanjšali koncentracije 4MMP, na vsebnost 3MH ta ukrep ni vplival.
Tudi s senzorično oceno vina smo potrdili pomembne vplive razlistanja pred in po cvetenju na določene note v aromi vina 'Sauvignon blanc'.
Leaf removal changes cluster microclimate, and thus modifications in the secondary metabolism are triggered, but differently as regard to the timing of application. In case of white grape varieties, the interest on the modification of aroma characteristics is searched, and in case of 'Sauvignon blanc', the shift in concentration of methoxypyrazines and thiols is responsible for the bouquet of the wines.
An experimental trial was set up in Oslavia (Gorizia, Italy) with the aim to evaluate how pre-flowering and post-flowering leaf removal applied on 'Sauvignon blanc' vines could change the aromatic occurrence in grapes and wines.
The yield parameters were slightly affected by the treatments of leaf removal; while late leaf removal did not impact on production, pre-flowering leaf removal slightly promoted a reduction on yield and cluster weight. Also basic maturation parameters were slightly changed, mainly higher soluble solids in case of pre-flowering leaf removal.
As regard aromatics, both thiol precursors in grapes and thiols in wines revealed to be shifted in case of pre-flowering leaf removal, with higher values of 4-mercapto-4-methyl-pentan-2-one and lower values of 3-mercaptohexan-1-ol. In case of late leaf removal lower concentration of 4MMP were revealed and similar of 3MH.
Also the degustation of the wines revealed some peculiarities of pre-flowering and late leaf removal treatments for some important sensorial notes of 'Sauvignon blanc' wines. Keywords: Vitis vinifera L. cv. 'Sauvignon blanc', razlistanje pred cvetenjem, razlistanje po cvetenju, aromatske komponente, tioli, LC-MS
Vitis vinifera L. cv. 'Sauvignon blanc', pre-flowering leaf removal, post-flowering leaf removal, aromatic compounds, thiols, LC-MS Published in RUNG: 14.01.2016; Views: 8913; Downloads: 307 Full text (1,30 MB) |
10. Vpliv vinogradniških tehnologij na vsebnost metabolitov v grozdju sorte ‘Modra frankinja’Ana Marija Tomše, 2015, undergraduate thesis Abstract: V grozdju sorte ‘Modra frankinja’ (Vitis vinifera L.), vinorodni okoliš Dolenjska, smo
leta 2013 preučevali vpliv vinogradniških tehnologij: odstranjevanja listov v predelu
grozdja (razlistanja) pred fazo cvetenja in redčenja grozdja v fazi vèraison v primerjavi
s kontrolo. Grozdje smo vzorčili teden pred trgatvijo, v času trgatve in teden po tem. V
svežem grozdnem soku smo določili vsebnost suhe snovi, skupnih titrabilnih kislin in
pH-vrednost. Vsebnost in profil antocianov smo določili v metanolnih ekstraktih
grozdnih kožic s pomočjo tekočinske kromatografije visoke ločljivosti. Redčenje
grozdja v fazi vèraison je vplivalo na statistično značilno večje povprečne: mase kožic,
vsebnosti suhe snovi in pH-vrednosti v primerjavi z razlistanjem pred fazo cvetenja ob
času trgatve in teden po tem, medtem ko se kontrola ni statistično značilno razlikovala
od redčenja grozdja. Razlike v povprečni masi grozdja in vsebnosti skupnih titrabilnih
kislin med tehnologijami niso bile statistično značilne ob nobenem terminu vzorčenja.
Povprečna vsebnost skupnih monomernih antocianov v grozdju je bila ob času trgatve
največja pri redčenju grozdja v fazi vèraison, vendar razlike med tehnologijami niso bile
statistično značilne. Malvidin 3-glukozid je najbolj zastopan antocian pri sorti ‘Modra
frankinja’. Lastnost sorte je tudi manjša vsebnost acilatov od kumaratov. Redčenje
grozdja v fazi vèraison je najbolj vplivalo na izboljšano kakovost grozdja glede na
spremljane parametre. Keywords: ‘Modra frankinja’ (Vitis vinifera L.), odstranjevanje listov pred fazo
cvetenja, redčenje grozdja v fazi vèraison, antociani, profil antocianov. Published in RUNG: 15.09.2015; Views: 8915; Downloads: 313 Full text (3,95 MB) |