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1.
Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertation
Gbenga Folorunsho Oginni, 2024, doctoral dissertation

Abstract: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Keywords: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Published in RUNG: 10.09.2024; Views: 620; Downloads: 11
.pdf Full text (3,80 MB)

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Development of strategies and molecular tools for obtaining application-friendly recombinant ligands
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, adhirons, phage display technology
Published in RUNG: 27.02.2023; Views: 1741; Downloads: 0
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Nanobodies as a flexible biotechnological platform
Ario De Marco, invited lecture at foreign university

Keywords: nanobodies, phage display, crystallography chaperones, functionalization strategies
Published in RUNG: 10.11.2019; Views: 3742; Downloads: 0
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6.
Nanobodies: How to Exploit Pre-Immune Libraries and Recombinant Antibody Fragments
Ario De Marco, unpublished invited conference lecture

Keywords: nanobodies, in vitro panning, phage display
Published in RUNG: 23.08.2019; Views: 4035; Downloads: 0
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Heptapeptides as potential neutralizers for Shiga toxin 1 cytotoxicity.
Robert Alvin Bernedo-Navarro, Mayara Mayele Miyachiro, Yano Tomomasa, unpublished conference contribution

Keywords: Shiga toxins, peptide phage display, neutralizers
Published in RUNG: 05.06.2019; Views: 4569; Downloads: 0
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Phage display and Shiga toxin neutralizers
Robert Alvin Bernedo Navarro, Tomomasa Yano, 2016, review article

Keywords: Shiga toxins, phage display
Published in RUNG: 27.05.2019; Views: 3800; Downloads: 0
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