1. Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertationGbenga Folorunsho Oginni, 2024, doctoral dissertation Abstract: Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Keywords: cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Published in RUNG: 10.09.2024; Views: 620; Downloads: 11
 Full text (3,80 MB) |
2. |
3. Native llama nanobody library panning performed by phage and yeast display provides binders suitable for C-reactive protein detectionSandra Oloketuyi, Robert Alvin Bernedo-Navarro, Andreas Christmann, Justyna Borkowska, Giulia Cazzaniga, Horst Wilhelm Schuchmann, Joanna Niedziółka-Jönsson, Katarzyna Szot-Karpińska, Harald Kolmar, Ario De Marco, 2021, original scientific article Keywords: phage display, yeast display, nanobodies, electrochemical impedance biosensors
Published in RUNG: 06.12.2021; Views: 2545; Downloads: 42
 Link to full text
This document has many files! More... |
4. Nanobody-dependent detection of microcystis aeruginosa by ELISA and thermal lens spectrometryGbenga Folorunsho Oginni, Sandra Oloketuyi, Elisa Mazzega, Hanna Budasheva, Alfred Beran, Marina Cabrini, Dorota Korte, Mladen Franko, Ario De Marco, 2021, original scientific article Keywords: cyanobacteria, nanobodies, fluobodies, phage display, thermal lens spectrometry
Published in RUNG: 19.04.2021; Views: 3116; Downloads: 0
This document has many files! More... |
5. |
6. |
7. |
8. |
9. |
10. Peptides derived from phage display libraries as potential neutralizers of Shiga toxin-induced cytotoxicity in vitro and in vivoRobert Alvin Bernedo Navarro, Mayara Miyachiro, Márcio da Silva, Rogério Conceição, Silvia Gatti, Tomomasa Yano, 2014, original scientific article Keywords: Shiga toxins, phage display, Stx1, Stx2, neutralizers
Published in RUNG: 27.05.2019; Views: 4521; Downloads: 0
This document has many files! More... |
