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1.
In vitro isolation of anti Alexandrium minutum nanobodies from a pre-immune library
Ario De Marco, Marina Cabrini, Alfred Beran, Elisa Mazzega, 2017, published scientific conference contribution abstract

Found in: ključnih besedah
Summary of found: ...nanobodies, toxic microalgae, biopanning...
Keywords: nanobodies, toxic microalgae, biopanning
Published: 04.12.2017; Views: 2896; Downloads: 18
.pdf Fulltext (61,76 KB)
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2.
Nanobodies as convenient reagents for multiple biotechnological applications
Ario De Marco, invited lecture at foreign university

Found in: ključnih besedah
Summary of found: ...nanobodies, immunodiagnostics, toxic microalgae...
Keywords: nanobodies, immunodiagnostics, toxic microalgae
Published: 19.10.2018; Views: 1859; Downloads: 0
.pdf Fulltext (813,75 KB)

3.
In vitro isolation of nanobodies for selective Alexandrium minutum recognition: a model for convenient development of dedicated immuno-reagents to study and diagnostic toxic unicellular algae
Elisa Mazzega, Alfred Beran, Marina Cabrini, Ario de Marco, 2019, original scientific article

Found in: ključnih besedah
Summary of found: ...toxic microalgae, immunoreagents, nanobodies, IF...
Keywords: toxic microalgae, immunoreagents, nanobodies, IF
Published: 07.01.2019; Views: 2327; Downloads: 0
.pdf Fulltext (2,35 MB)

4.
Nutritional quality and safety of the spirulina dietary supplements sold on the Slovenian market
Nives Ogrinc, Iztok Arčon, Marijan Nečemer, Katarina Vogel-Mikuš, Marta Jagodic Hudobivnik, Jasmina Masten, 2022, original scientific article

Abstract: The microalgae Spirulina may be a popular dietary supplement rich in essential nutrients and vitamins, but oversight of the supplement industry, in general, remains limited, and increasing incidents of adulteration, misbranding, and undeclared ingredients together with misleading claims create potential risks. In response, this study characterized the elemental, amino acid and fatty acid content of commercially available Spirulina supplements in Slovenia using EDXRF, ICP-MS and GC-MS and compared the results with their nutritional declaration. The gathered data confirm that Spirulina supplements are a good source of calcium (0.15 to 29.5% of RDA), phosphorous (3.36–26.7% of RDA), potassium (0.5 to 7.69% of RDA) and selenium (0.01 to 38.6% of RDA) when consumed within recommended amounts. However, although iron contents were relatively high (7.64 to 316% of RDA), the actual bioavailability of iron was much lower since it was mainly present as the ferric cation. This study also confirms that pure Spirulina supplements are a good source of essential and non-essential amino acids, and !-6 but not !-3 polyunsaturated fatty acids. The presence of additives resulted in significant variation in nutrient content and, in some instances, lower product quality. Moreover, a high proportion (86.7%) of inappropriate declarations regarding the elemental content was observed. Overall, the study conclusions underline the need for a stricter control system for Spirulina-based supplements.
Found in: ključnih besedah
Summary of found: ...Spirulina, microalgae, cyanobacteria, elements, toxic elements, amino acids, fatty acids, authenticity, safety, quality...
Keywords: Spirulina, microalgae, cyanobacteria, elements, toxic elements, amino acids, fatty acids, authenticity, safety, quality
Published: 24.03.2022; Views: 345; Downloads: 0
.pdf Fulltext (1,26 MB)

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FUNCTIONALIZED NANOBODIES AS DIAGNOSTIC REAGENTS FOR THE DETECTION OF TOXIC MICROALGAE
Sandra Oloketuyi, 2020, doctoral dissertation

Abstract: Early detection and monitoring of toxic microalgae remains a challenge that will be necessary to overcome for understanding harmful algal bloom events and their consequent ecological and environmental impacts. Although there are diagnostic techniques based on microscopy, fluorescence in situ hybridization (FISH), DNA microarrays, real-time PCR, sandwich hybridization assays employed for the detection of harmful algal species, such techniques are not suitable for field monitoring and especially for the quantification of Alexandrium minutum due to abundance of cryptic species that cannot be differentiated based on morphology or genomic traits. These approaches are also time-consuming and laborious, hence there is need for alternative simple, rapid and cost-effective methods. In this study, our aim was to develop different nanobody-based capture strategies for the detection of A. minutum. Nanobodies were successfully displayed on the surface of Escherichia coli to facilitate detection of A. minutum. The method is simple and cost effective as no antibody purification step is necessary. As an alternative, A. minutum was quantified by exploiting the peroxidase activity of a G-quadruplex DNAzyme covalently bound to nanobodies and by means of a label free electrochemical immunosensor which exploit nanobodies bound via an irreversible SpyTag-SpyCatcher system as the specific immunocapture element. Such immunosensor was prepared by immobilizing the SpyTagged anti-A. minutum nanobody (SpyTagged C1) by means of a selfassembled monolayer (SAMs) of L- cysteine (L-Cys) displayed onto a gold nanoparticle-modified glassy carbon electrode. Electrochemical Impedance Spectroscopy (EIS) was performed to measure the electrical response of the circuit as the function of A. minutum immunocaptured cells. The results of EIS studies confirmed that the sensing chip detected A. minutum selectively, exhibited a wide dynamic range spanning from 103 to 109 cell L-1 and a limit of detection of 3.1× 103 cell L-1. The immunosensor data can be recorded and then analyzed with a portable potentiostat. Such device can be interfaced with a smart phone, a condition suitable for the rapid A. minutum quantification in situ.
Found in: ključnih besedah
Keywords: Alexandrium minutum, nanobodies, bioreagents, immunosensor, toxic microalgae
Published: 17.06.2020; Views: 2368; Downloads: 137
.pdf Fulltext (2,95 MB)

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