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Title:Microfluidic flow injection thermal lens microscopy for high throughput and sensitive analysis of sub-μL samples
Authors:ID Liu, Mingqiang, UNG (Author)
ID Malovrh, Sara, UNG (Author)
ID Franko, Mladen, UNG (Author)
Files:.pdf Cr(VI).pdf (828,33 KB)
MD5: DCC2EA043B403CDADC4349647FD888D3
 
Language:English
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:An analytical method combining microfluidic flow injection analysis (μFIA) with thermal lens microscopy (TLM) was developed for high throughput and sensitive analysis of sub-μL samples. Performance of the μFIA-TLM was validated for detection of hexavalent chromium [Cr(VI)] in water samples. At different sample injection volumes, detection positions and flow rates, influences of the reaction time and the diffusion of Cr-diphenylcarbazone (DPCO) complexes on the μFIA-TLM signal were investigated. Photodegradation of the Cr-DPCO complex was clearly observed when the absorbed photons per Cr-DPCO is above 1600. After optimization of the TLM with respect to rapid flows (up to 10 cm/s), we achieved a limit of detection of 0.6 ng/mL for Cr(VI) in a 50-μm deep channel. Impacts of interfering ions [V(V), Mo(VI), Fe(III)] on the Cr(VI) determination were found to be small. Cr(VI) in real samples from a cement factory were determined and found in good agreement with results of spectrophotometry. This μFIA-TLM shows advantages over its conventional counterpart, such as eliminating additional sample conditioning, reducing over 100 times the sample consumption to sub-μL and over 10 times the time required for one sample injection to a few seconds (up to 20 samples/min). The optimized μFIA-TLM setup can be applied for fast and sensitive analysis of nonfluoresent sub-μL samples in rapidly flowing mediums.
Keywords:Spektrometrija TLS, mikroskopija TLM, mikrofluidika, krom (VI)
Publication version:Author Accepted Manuscript
Year of publishing:2016
Number of pages:8
Numbering:8
PID:20.500.12556/RUNG-2328 New window
COBISS.SI-ID:4365051 New window
DOI:10.1039/C6AY00932H New window
NUK URN:URN:SI:UNG:REP:GJHFAC3A
Publication date in RUNG:01.06.2016
Views:6593
Downloads:277
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LIU, Mingqiang, MALOVRH, Sara and FRANKO, Mladen, 2016, Microfluidic flow injection thermal lens microscopy for high throughput and sensitive analysis of sub-μL samples. Analytical methods [online]. 2016. Vol. 8. [Accessed 4 April 2025]. DOI 10.1039/C6AY00932H. Retrieved from: https://repozitorij.ung.si/IzpisGradiva.php?lang=eng&id=2328
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Record is a part of a journal

Title:Analytical methods
Shortened title:Anal. Methods
Year of publishing:2016
ISSN:ISSN 0003-2654

Document is financed by a project

Funder:Other - Other funder or multiple funders

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