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Title:Bilirubin is an Endogenous Antioxidant in Human Vascular Endothelial Cells
Authors:ID Žiberna, Lovro, Univerza v Trstu (Author)
ID Martelanc, Mitja, Univerza v Novi Gorici (Author)
ID Franko, Mladen, Univerza v Novi Gorici (Author)
ID Passamonti, Sabina, Univerza v Trstu (Author)
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Language:English
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Bilirubin is a standard serum biomarker of liver function. Inexplicably, it is inversely correlated with cardiovascular disease risk. Given the role of endothelial dysfunction in originating cardiovascular diseases, direct analysis of bilirubin in the vascular endothelium would shed light on these relationships. Hence, we used high-performance liquid chromatography coupled with thermal lens spectrometric detection and diode array detection for the determination of endogenous cellular IXα-bilirubin. To confirm the isomer IXα-bilirubin, we used ultra-performance liquid chromatography coupled with a high-resolution mass spectrometer using an electrospray ionization source, as well as tandem mass spectrometric detection. We measured bilirubin in both arterial and venous rat endothelium (0.9–1.5 pmol mg−1 protein). In the human endothelial Ea.hy926 cell line, we demonstrated that intracellular bilirubin (3–5 pmol mg−1 protein) could be modulated by either extracellular bilirubin uptake, or by up-regulation of heme oxygenase-1, a cellular enzyme related to endogenous bilirubin synthesis. Moreover, we determined intracellular antioxidant activity by bilirubin, with EC50 = 11.4 ± 0.2 nM, in the range of reported values of free serum bilirubin (8.5–13.1 nM). Biliverdin showed similar antioxidant properties as bilirubin. We infer from these observations that intra-endothelial bilirubin oscillates, and may thus be a dynamic factor of the endothelial function.
Keywords:Antioxidants, bilirubin, endothelial cells
Publication version:Version of Record
Year of publishing:2016
Number of pages:6
Numbering:6, 29240
PID:20.500.12556/RUNG-2490 New window
COBISS.SI-ID:4431099 New window
DOI:10.1038/srep29240 New window
NUK URN:URN:SI:UNG:REP:LMXAR9KU
Publication date in RUNG:07.07.2016
Views:6295
Downloads:409
Metadata:XML DC-XML DC-RDF
:
ŽIBERNA, Lovro, MARTELANC, Mitja, FRANKO, Mladen and PASSAMONTI, Sabina, 2016, Bilirubin is an Endogenous Antioxidant in Human Vascular Endothelial Cells. Scientific Reports [online]. 2016. Vol. 6, no. 29240. [Accessed 17 April 2025]. DOI 10.1038/srep29240. Retrieved from: https://repozitorij.ung.si/IzpisGradiva.php?lang=eng&id=2490
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Record is a part of a journal

Title:Scientific Reports
Shortened title:Sci. Rep.
Publisher:Nature Publishing Group: Open Access Journals - Option C
Year of publishing:2016
ISSN:2045-2322

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