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Title:EFFECT OF DIET ON HAIR CORTISOL AND DHEA CONCENTRATIONS IN MOUSE
Authors:ID Montillo, Marta (Copyright holder)
ID Comin, Antonella, University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences (Author)
ID Montillo, Marta, University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences (Author)
ID Peric, Tanja, University of Nova Gorica, Center for Biomedical Science and Engineering (Author)
ID Gazzin, Silvia, AREA science park Basovizza, Centro Studi Fegato (Author)
ID Corazzin, Mirco, University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences (Author)
ID Prandi, Alberto, University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences (Author)
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Language:English
Work type:Not categorized
Typology:1.12 - Published Scientific Conference Contribution Abstract
Organization:UNG - University of Nova Gorica
Abstract:Obesity and MetS (Metabolic Syndrome) are both linked to persistent long-term hormonal and metabolic changes. In most of the studies, cortisol (C) and dehydroepiandrosterone (DHEA) concentrations have been measured in obese and normal-weight subjects, obtaining heterogeneous results. Plasma, saliva and urine, matrices that represent timepoint or short-term steroids exposure, were used for these studies. The aim was to study C, DHEA and C/DHEA ratio of mice pups in the hair, matrix capable of providing cumulative hormonal exposure. Sixty C57Bl/6 mice pups (30 males and 30 females) were housed in a temperature-controlled environment (22±2°C) and on a 12h light/dark schedule, under ad-libitum access to food and water for 16 weeks (welfare: Italian Law Decree 116-92 and EC Directive 86-609-EEC). Control and experimental diets were offered immediately after weaning (3 weeks old pups), for 16 weeks. Four experimental checkpoints were established (T1: 4 weeks, T2: 8 weeks, T3: 12 weeks and T4: 16 weeks of diet). T3 and T4 showed the hormonal concentrations of pubertal animals. Twentyseven pups (13 females and 14 males) were randomly group-housed in cages (6 for T1, 5 for T2, 6 for T3, 10 for T4) and assigned to control diet (CTRL: D12328, Research Diets, New Brunswick, NJ). 33 pups (17 females and 16 males) were randomly group-housed in cages (5 for T1, 6 for T2 and T3, 16 for T4) and assigned to the HFHC diet (HFHC: D12331, Research Diets, New Brunswick, NJ - plus 42g/L fructose/sucrose in drinking water). At each experimental checkpoint, all the animals of one cage for each experimental group were suppressed. Hair strands were carefully cut with scissors as close as possible to the skin from the back of the mice, paying attention to not to wound the animals. Hair was stored in an envelope at RT in a dry room until use. C and DHEA hair concentrations was measured by a solid-phase microtiter RIA assay (Peric et al., 2016 adapted in the mouse). Only at 8 weeks the HFHC group showed significantly higher C concentrations than the CTRL group (1.56±0.06 vs 1.92±0.130 pg/mg; P<0.05). DHEA concentrations were significantly reduced in the HFHC group than the CTRL group at 4 (114.64±13.93 vs 69.08±5.33 pg/mg; P<0.05), 8 (71.67±7.08 vs 50.38±4.74 pg/mg; P<0.05), 12 (73.27±8.29 vs 41.59±1.60 pg/mg; P<0.01) and 16 weeks (65.26±3.35 vs 55.05±1.84 pg/mg; P<0.05). The C/DHEA ratio was significantly increased in the HFHC than the CTRL group, at 8 (0.023±0.002 vs 0.040±0.005; P<0.01), 12 (0.024±0.003 vs 0.036±0.004; P<0.05) and 16 weeks (0.019±0.001 vs 0.025±0.002; P<0.01). The gender effect was not significant. Taking into account the lag time required for the hair emersion from the skin (about 1 week), the significant stimulation of the C at 8 weeks in the HFHC group refers to its chronic elevation from 5 to 7 weeks of treatment when the animals were not pubertal. Conversely, DHEA shows a chronic reduction in obese mice leading to consider an independent adrenal regulation of C and DHEA, both stimulated by ACTH. Research was supported by the Project CBM (MIUR DM60643). Peric et al. 2016 J Appl Anim Welf Sci 18:1-8.
Keywords:DIET, HAIR, CORTISOL, DHEA, MOUSE
Publication status:Published
Number of pages:2
PID:20.500.12556/RUNG-2521 New window
COBISS.SI-ID:4445179 New window
NUK URN:URN:SI:UNG:REP:EINAQLKW
Publication date in RUNG:21.07.2016
Views:5916
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Document is financed by a project

Funder:Other - Other funder or multiple funders
Project number:MIUR DM60643
Name:Nutrizione e Salute - Dalla nutrigenetica alla nutraceutica: sviluppo di azioni sinergiche ed integrate per la realizzazione di test, diete e prodotti in grado di migliorare la salute pubblica e prevenire le principali patologie alimentari

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