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Title:Biotinylated Vanadium and Chromium Sulfide Nanoparticles as Probes for Colocalization of Membrane Proteins
Authors:ID Loukanov, Alexandre, Graduate School of Science and Engineering, Saitama University, Japan (Author)
ID Emin, Saim, University of Nova Gorica, Slovenia (Author)
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Language:Slovenian
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:ABSTRACT We report the microemulsion synthesis of vanadium and chromium sulfide nanoparticles (NPs) and their biological application as nanoprobes for colocalization of membrane proteins. Spherical V2S3 and Cr2S3 NPs were prepared in reverse microemulsion droplets, as nanoreactors, obtained by the surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in nonpolar organic phase (heptane). Electron microscopic data indicated that the size distribution of the nanoparticles was uniform with an average diameter between 3 4 5 nm. The prepared hydrophobic nanocrystals were transferred in aqueous phase by surface cap exchange of AOT with biotindihydrolipoic ligands. This substitution allows the nanoparticles solubility in aqueous solutions and confer their bioactivity. In addition, we report the conjugation procedure between a-Lipoic acid (LA) and biotin (abbreviated as biotin-LA). The biotin-LA structure was characterized by 1D and 2D NMR spectroscopy. The biotinylated vanadium and chromium sulfide nanoparticles were tested as probes for colocalization of glutamate receptors on sodium-dodecyl-sulfate-digested replica prepared from rat hippocampus. The method suggests their high labeling efficiency for study of membrane biological macromolecules.
Keywords:biotin-dihydrolipoic conjugate, vanadium and chromium sulfide nanoparticles, membrane proteins
Year of publishing:2016
Number of pages:79
Numbering:2016, DOI: 10.1002/jemt.22701
PID:20.500.12556/RUNG-2937-56933765-3b00-f770-d92d-bf50539a9c8c New window
COBISS.SI-ID:4655099 New window
DOI:10.1002/jemt.22701 New window
NUK URN:URN:SI:UNG:REP:U8CKUTQW
Publication date in RUNG:02.02.2017
Views:5977
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Record is a part of a journal

Title:MICROSCOPY RESEARCH AND TECHNIQUE
Year of publishing:2016

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.02.2017

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