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Title:An extracellular polymeric substance quickly chelates mercury(II) with N-heterocyclic groups
Authors:ID Baldi, Franco, University Cà Foscari Venezia (Author)
ID Gallo, Michele, University Cà Foscari Venezia (Author)
ID Daniale, Salvatore, University Cà Foscari Venezia (Author)
ID Battistel, Dario, University Cà Foscari Venezia (Author)
ID Faleri, Claudia, Siena University (Author)
ID Kodre, Alojz, Univerza v Ljubljani (Author)
ID Arčon, Iztok, Univerza v Novi Gorici (Author)
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Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:A strain of Klebsiella oxytoca DSM 29614 is grown on sodium citrate in the presence of 50 mg l−1 of Hg as Hg(NO3)2. During growth, the strain produces an extracellular polymeric substance (EPS), constituted by a mixture of proteins and a specific exopolysaccharide. The protein components, derived from the outer membrane of cells, are co-extracted with the extracellular exopolysaccharide using ethanol. The extracted EPS contains 7.5% of Hg (total amount). This indicates that EPS is an excellent material for the biosorption of Hg2+, through chemical complexation with the EPS components. The binding capacity of these species towards Hg2+ is studied by cyclic voltammetry, and Hg L3-edge XANES and EXAFS spectroscopy. The results found indicate that Hg2+ is mainly bound to the nitrogen of the imidazole ring or other N-heterocycle compounds. The hydroxyl moities of sugars and/or the carboxyl groups of two glucuronic acids in the polysaccharide can also play an important role in sequestring Hg2+ ions. However, N-heterocyclic groups of proteins bind Hg2+ faster than hydroxyl and carboxyl groups of the polysaccharide.
Keywords:Klebsiella oxytoca, Biosorption, Proteins, Polysaccharide, Cyclic voltammetry, EXAFS analysis
Publication version:Author Accepted Manuscript
Year of publishing:2017
Number of pages:10
Numbering:February 2017
PID:20.500.12556/RUNG-3004-5ba07d23-9418-50e3-4a4b-24546cf64098 New window
COBISS.SI-ID:4706811 New window
DOI:10.1016/j.chemosphere.2017.02.093 New window
Publication date in RUNG:03.03.2017
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Year of publishing:2017