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Title:Whole-cell biopanning with a synthetic phage display library of nanobodies enabled the recovery of follicle-stimulating hormone receptor inhibitors
Authors:ID Crepin, Ronan, Institut Curie (Author)
ID Veggiani, Gianluca, Institut Curie (Author)
ID Djender, Selma, Institut Curie (Author)
ID Beugnet, Anne, Institut Curie (Author)
ID Planeix, Francois, Institut Curie (Author)
ID Pichon, Christophe, Institut Curie (Author)
ID Moutel, Sandrine, Institut Curie (Author)
ID Amigorena, Sebastian, Institut Curie (Author)
ID Pérez, Franck, Institut Curie (Author)
ID Ghinea, Nicolae, Institut Curie (Author)
ID De Marco, Ario, UNG (Author), et al.
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Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm. We expect that the proposed biopanning strategy will be profitable to identify useful functional antibodies for further members of the GPCR class.
Keywords:nanobodies, GPCR proteins, FSHR, panning, pre-immune antibody library
Publication version:Version of Record
Year of publishing:2017
Number of pages:1567-1572
PID:20.500.12556/RUNG-3322-b86c7cca-992c-0d39-70bc-b8e3cc19c7ad New window
COBISS.SI-ID:4933627 New window
Publication date in RUNG:19.10.2017
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Title:Biochemical Biophysical Research Communications


License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:19.10.2017