| Title: | Hemichrome Determination by Thermal Lensing with Polyethylene Glycols for Signal Enhancement in Aqueous Solutions |
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| Authors: | ID Galimova, Viktoriya, Lomonosov Moscow State University (Author)
ID Franko, Mladen, Univerza v Novi Gorici (Author)
ID Proskurnin, Mikhail, Lomonosov Moscow State University (Author)
ID Liu, Mingqiang, Universe v Novi Gorici (Author), et al. |
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| Files: |
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| Language: | English |
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| Work type: | Not categorized |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | UNG - University of Nova Gorica
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| Abstract: | The thermal lens technique is proposed for the determination of total hemoglobin in the form of reversible hemichrome. The conditions were optimized (concentration of sodium dodecyl sulfate, 2 mM) to attain the maximum sensitivity with the use of polyethylene glycols as signal enhancers. For polyethylene glycols with molecular weights 1500–35000 Da in a concentration range of 5–15% w/w (5–25 mM), the influence on thermal lens signal enhancement was estimated. It is shown that the use of 5% w/w polyethylene glycol 2000 provides the maximum increase in the thermal lens enhancement factor (by 40%) in comparison with unmodified aqueous solutions. The detection limit of iron(II) tris(1,10-phenanthrolinate) as a model system is 60 nM. Under these conditions, the thermal lens detection limit of hemichrome is 10 nM, which shows a 15-fold enhancement compared to spectro- photometry. Modification of the medium with polyethylene glycols decreases the limit of detection of hemichrome determination by 15% in comparison with unmodified aqueous solutions due to better reproducibility for the range of concentrations from 0.02 to 0.9 μM. |
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| Keywords: | Hemoglobin, hemikrom, optotermična spektroskopija, etilenglikol, spektrometrija s toplotnimi lečami |
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| Publication version: | Submitted Version |
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| Year of publishing: | 2018 |
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| Number of pages: | 1743-1762 |
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| Numbering: | 51, 11 |
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| PID: | 20.500.12556/RUNG-4081 |
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| COBISS.SI-ID: | 5136891 |
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| DOI: | 10.1080/00032719.2017.1391828 |
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| NUK URN: | URN:SI:UNG:REP:LX3R9ASK |
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| Publication date in RUNG: | 16.04.2018 |
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| Views: | 4967 |
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| Downloads: | 0 |
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