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Title:Phosphorylation of HPV-16 L2 Contributes To Efficient Virus Infectious Entry
Authors:ID Broniarczyk, Justyna (Author)
ID Massimi, Paola (Author)
ID Pim, David, ICGEB (Author)
ID Bergant Marušič, Martina (Author)
ID Myers, Michael P. (Author)
ID Garcea, Robert L. (Author)
ID Banks, Lawrence (Author)
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Language:English
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:The Human Papillomavirus (HPV) capsid comprises two viral proteins, L1 and L2, with the L2 component being essential to ensure efficient endocytic transport of incoming viral genomes. Several studies have previously reported that L1 and L2 are post-translationally modified, but it is uncertain whether these modifications affect HPV infectious entry. Using a proteomic screen, we identified a highly conserved phospho-acceptor site on the HPV-16 and BPV-1 L2 proteins. The phospho-modification of L2, and its presence in HPV pseudovirions (PsVs), was confirmed using anti-phospho L2-specific antibodies. Mutation of the phospho-acceptor sites of both HPV-16 and BPV-1 L2 resulted in the production of infectious virus particles, with no differences in efficiency of packaging the reporter DNA. However, these mutated PsVs showed marked defects in infectious entry. Further analysis revealed a defect in uncoating, characterized by a delay in the exposure of a conformational epitope on L1 that indicates capsid uncoating. This uncoating defect was accompanied by a delay in the proteolysis of both L1 and L2 in mutated HPV-16 PsVs. Taken together, these studies indicate that phosphorylation of L2 during virus assembly plays an important role in optimal uncoating of virions during infection, suggesting that phosphorylation of the viral capsid proteins contributes to infectious entry.
Keywords:HPV, L2, infection, protein phosphorylation
Publication version:Submitted Version
Year of publishing:2019
Number of pages:14
Numbering:0
PID:20.500.12556/RUNG-4565-6b4290bd-3e16-077b-159f-ce3035d65145 New window
COBISS.SI-ID:5402619 New window
DOI:10.1128/JVI.00128-19 New window
NUK URN:URN:SI:UNG:REP:EIBNRM1Y
Publication date in RUNG:05.06.2019
Views:3533
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Record is a part of a journal

Title:Journal of Virology
Year of publishing:2019

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:03.06.2019

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