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Title:Development of an adaptable headspace sampling method for metabolic profiling of the fungal volatome
Authors:ID Ahmed, Waqar M, School of Biological Sciences, University of Manchester, United Kingdom (Author)
ID Geranios, Pavlos, School of Biological Sciences, University of Manchester, United Kingdom (Author)
ID White, Iain R., School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, United Kingdom (Author)
ID Lawal, Oluwasola, School of Biological Sciences, University of Manchester, United Kingdom (Author)
ID Nijsen, Tamara M, Philips Research, Royal Philips B.V., Netherlands (Author)
ID Bromley, Michael J, School of Biological Sciences, University of Manchester, United Kingdom (Author)
ID Goodacre, Royston, School of Chemistry, Manchester Institute of Biotechnology, University of Manchester, United Kingdom (Author)
ID Read, Nicholas D, School of Biological Sciences, University of Manchester, United Kingdom (Author)
ID Fowler, Stephen J., School of Biological Sciences, University of Manchester, United Kingdom (Author)
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Language:English
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Pulmonary aspergillosis can cause serious complications in people with a suppressed immune system. Volatile metabolites emitted by Aspergillus spp. have shown promise for early detection of pathogenicity. However, volatile profiles require further research, as effective headspace analysis methods are required for extended chemical coverage of the volatome; in terms of both very volatile and semi-volatile compounds. In this study, we describe a novel adaptable sampling method in which fungal headspace samples can be sampled continuously throughout a defined time period using both active (pumped) and passive (diffusive) methods, with the capability for samples to be stored for later off-line analysis. For this method we utilise thermal desorption-gas chromatography-mass spectrometry to generate volatile metabolic profiles using Aspergillus fumigatus as the model organism. Several known fungal-specific volatiles associated with secondary metabolite biosynthesis (including α-pinene, camphene, limonene, and several sesquiterpenes) were identified. A comparison between the wild-type A. fumigatus with a phosphopantetheinyl transferase null mutant strain (ΔpptA) that is compromised in secondary metabolite synthesis, revealed reduced production of sesquiterpenes. We also showed the lack of terpene compounds production during the early growth phase, whilst pyrazines were identified in both early and late growth phases. We have demonstrated that the fungal volatome is dynamic and it is therefore critically necessary to sample the headspace across several time periods using a combination of active and passive sampling techniques to analyse and understand this dynamism.
Keywords:Volatile Organic Compounds, Fungi, Mycelial growth
Publication version:Version of Record
Year of publishing:2018
Number of pages:4155-4162
Numbering:17, 143
PID:20.500.12556/RUNG-4650-a846bb94-759d-eecd-8f2d-2031c1a432f6 New window
COBISS.SI-ID:5419771 New window
DOI:10.1039/C8AN00841H New window
NUK URN:URN:SI:UNG:REP:OTUOJWSA
Publication date in RUNG:18.07.2019
Views:3087
Downloads:0
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Record is a part of a journal

Title:Analyst
Shortened title:Analyst
Publisher:Royal Society of Chemistry
ISSN:0003-2654

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