| Title: | Hypoxia influences the cellular cross-talk of human dermal fibroblasts. A proteomic approach. |
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| Authors: | ID Federica, Boraldi, University of Modena and Reggio Emilia (Author)
ID Giulia, Annovi, University of Modena and Reggio Emilia (Author)
ID Fabio, Carraro, University of Siena (Author)
ID Antonella, Naldini, University of Siena (Author)
ID Roberta, Tiozzo, University of Modena and Reggio Emilia (Author)
ID Pascal, Sommer, Université Lyon I (Author)
ID Daniela, Quaglino, University of Modena and Reggio Emilia (Author), et al. |
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| Files: |
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| Language: | English |
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| Work type: | Not categorized |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | UNG - University of Nova Gorica
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| Abstract: | The ability of cells to respond to changes in oxygen availability is critical for many physiological and pathological processes (i.e. development,
aging, wound healing, hypertension, cancer). Changes in the protein profile of normal human dermal fibroblasts were investigated in vitro after
96 h in 5% CO2 and 21% O2 (pO2=140 mm Hg) or 2% O2 (pO2=14 mm Hg), these parameters representing a mild chronic hypoxic exposure
which fibroblasts may undergo in vivo. The proliferation rate and the protein content were not significantly modified by hypoxia, whereas
proteome analysis demonstrated changes in the expression of 56 proteins. Protein identification was performed by mass spectrometry. Data
demonstrate that human fibroblasts respond to mild hypoxia increasing the expression of hypoxia inducible factor (HIF1a) and of the 150-kDa
oxygen-regulated protein. Other differentially expressed proteins appeared to be related to stress response, transcriptional control, metabolism,
cytoskeleton, matrix remodelling and angiogenesis. Furthermore, some of them, like galectin 1, 40S ribosomal protein SA, N-myc-downstream
regulated gene-1 protein, that have been described in the literature as possible cancer markers, significantly changed their expression also in
normal hypoxic fibroblasts. Interestingly, a bovine fetuin was also identified that appeared significantly less internalised by hypoxic fibroblasts. In
conclusion, results indicate that human dermal fibroblasts respond to an in vitro mild chronic hypoxic exposure by modifying a number of
multifunctional proteins. Furthermore, data highlight the importance of stromal cells in modulating the intercellular cross-talk occurring in
physiological and in pathologic conditions. |
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| Keywords: | Human fibroblast, Primary cell culture, Hypoxia, Connective tissue, Proteome, 2D gel electrophoresis, Mass-spectrometry |
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| Year of publishing: | 2007 |
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| Number of pages: | 1402-1413 |
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| Numbering: | 1774 |
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| PID: | 20.500.12556/RUNG-4662-9db8e07b-5b5c-b597-fdde-e297308468a4 |
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| COBISS.SI-ID: | 5425147 |
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| NUK URN: | URN:SI:UNG:REP:YJMYQ6CC |
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| Publication date in RUNG: | 22.07.2019 |
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| Views: | 4310 |
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| Downloads: | 0 |
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