Repository of University of Nova Gorica

Show document
A+ | A- | Help | SLO | ENG

Title:The effect of serum withdrawal on the protein profile of quiescent human dermal fibroblasts in primary cell culture.
Authors:ID Federica, Boraldi, University of Modena and Reggio Emilia (Author)
ID Giulia, Annovi, University of Modena and Reggio Emilia (Author)
ID Chiara, Paolinelli Devincenzi, University of Modena and Reggio Emilia (Author)
ID Roberta, Tiozzo, University of Modena and Reggio Emilia (Author)
ID Daniela, Quaglino, University of Modena and Reggio Emilia (Author)
Files: This document has no files that are freely available to the public. This document may have a physical copy in the library of the organization, check the status via COBISS. Link is opened in a new window
Language:English
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:The effect of serum deprivation on proliferating cells is well known, in contrast its role on primary cell cultures, at confluence, has not been deeply investigated. Therefore, in order to explore the response of quiescent cells to serum deprivation, ubiquitous mesenchymal cells, as normal human dermal fibroblasts, were grown, for 48 h after confluence, in the presence or absence of 10% FBS. Fibroblast behaviour (i.e. cell morphology, cell viability, ROS production and elastin synthesis) was evaluated morphologically and biochemically. Moreover, the protein profile was investigated by 2-DE and differentially expressed proteins were identified by MS. Serum withdrawal caused cell shrinkage but did not significantly modify the total cell number. ROS production, as evaluated by the dihydroethidium (DH2) probe, was increased after serum deprivation, whereas elastin synthesis, measured by a colorimetric method, was markedly reduced in the absence of serum. By proteome analysis, 41 proteins appeared to significantly change their expression, the great majority of protein changes were related to the cytoskeleton, the stress response and the glycolytic pathway. Data indicate that human dermal fibroblasts in primary cell culture can adapt themselves to environmental changes, without significantly altering cell viability, at least after a few days of treatment, even though serum withdrawal represents a stress condition capable to increase ROS production, to influence cell metabolism and to interfere with cell behaviour, favouring the expression of several age-related features.
Keywords:Dermal fibroblasts / Primary cell culture / ROS production / Serum withdrawal
Year of publishing:2008
Number of pages:66-82
Numbering:8
PID:20.500.12556/RUNG-4663-ef5d1e98-1a19-1437-d28b-68f25a6fbb13 New window
COBISS.SI-ID:5425659 New window
NUK URN:URN:SI:UNG:REP:UXJGPMJW
Publication date in RUNG:22.07.2019
Views:3476
Downloads:0
Metadata:XML RDF-CHPDL DC-XML DC-RDF
:
Copy citation
  
Average score:(0 votes)
Your score:Voting is allowed only for logged in users.
Share:Bookmark and Share


Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Record is a part of a journal

Title:Proteomics
Year of publishing:2008

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:19.07.2019

Back