| Title: | New insights into autophagic cell death in the gypsy moth Lymantria dispar: a proteomic approach. |
|---|
| Authors: | ID Malagoli, Davide, Università di Modena e Reggio Emilia (Author)
ID Boraldi, Federica, Università di Modena e Reggio Emilia (Author)
ID Annovi, Giulia, Università di Modena e Reggio Emilia (Author)
ID Quaglino, Daniela, Università di Modena e Reggio Emilia (Author)
ID Ottaviani, Enzo, Università di Modena e Reggio Emilia (Author) |
|---|
| Files: |
This document has no files that are freely available to the public. This document may have a physical copy in the library of the organization, check the status via COBISS.  |
|---|
| Language: | English |
|---|
| Work type: | Not categorized |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: | UNG - University of Nova Gorica
|
|---|
| Abstract: | Autophagy is an evolutionary ancient process based on the activity of genes conserved from yeast to metazoan taxa. Whereas its role as a mechanism to provide energy during cell starvation is commonly accepted, debate continues about the occurrence of autophagy as a means specifically activated to achieve cell death. The IPLB-LdFB insect cell line, derived from the larval fat body of the lepidoptera Lymantria dispar, represents a suitable model to address this question, as both autophagic and apoptotic cell death can be induced by various stimuli. Using morphological and functional approaches, we have observed that the culture medium conditioned by IPLB-LdFB cells committed to death by the ATPase inhibitor oligomycin A stimulates autophagic cell death in untreated IPLB-LdFB cells. Moreover, proteomic analysis of the conditioned media suggests that, in IPLB-LdFB cells, oligomycin A promotes a shift towards lipid metabolism, increases oxidative stress and specifically directs the cells towards autophagic activity. |
|---|
| Keywords: | Autophagic cell death, Fat body, IDGF, IPLB-LdFB, Proteomics |
|---|
| Year of publishing: | 2009 |
|---|
| Number of pages: | 107-118 |
|---|
| Numbering: | 336 |
|---|
| PID: | 20.500.12556/RUNG-4692-da392a29-5d28-1aa4-a42a-997984f38319 |
|---|
| COBISS.SI-ID: | 5439227 |
|---|
| DOI: | 10.1007/s00441-008-0748-8 |
|---|
| NUK URN: | URN:SI:UNG:REP:IGMCPL3D |
|---|
| Publication date in RUNG: | 23.08.2019 |
|---|
| Views: | 3753 |
|---|
| Downloads: | 0 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Average score: | (0 votes) |
|---|
| Your score: | Voting is allowed only for logged in users. |
|---|
| Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
