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Title:Phosphate availability and ectomycorrhizal symbiosis with Pinus sylvestris have independent effects on the Paxillus involutus transcriptome
Authors:ID Paparokidou, Christina, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK (Author)
ID Leake, Jonathan R., Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK (Author)
ID Beerling, David J., Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK (Author)
ID Rolfe, Stephen A., Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK (Author)
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Language:English
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Many plant species form symbioses with ectomycorrhizal fungi, which help them forage for limiting nutrients in the soil such as inorganic phosphate (Pi). The transcriptional responses to symbiosis and nutrient-limiting conditions in ectomycorrhizal fungal hyphae, however, are largely unknown. An artificial system was developed to study ectomycorrhizal basidiomycete Paxillus involutus growth in symbiosis with its host tree Pinus sylvestris at different Pi concentrations. RNA-seq analysis was performed on P. involutus hyphae growing under Pi-limiting conditions, either in symbiosis or alone. We show that Pi starvation and ectomycorrhizal symbiosis have an independent effect on the P. involutus transcriptome. Notably, low Pi availability induces expression of newly identified putative high-affinity Pi transporter genes, while reducing the expression of putative organic acid transporters. Additionally, low Pi availability induces a close transcriptional interplay between P and N metabolism. GTP-related signalling was found to have a positive effect in the maintenance of ectomycorrhizal symbiosis, whereas multiple putative cytochrome P450 genes were found to be downregulated, unlike arbuscular mycorrhizal fungi. We provide the first evidence of global transcriptional changes induced by low Pi availability and ectomycorrhizal symbiosis in the hyphae of P. involutus, revealing both similarities and differences with better-characterized arbuscular mycorrhizal fungi.
Keywords:Ectomycorrhizal symbiosis, Paxillus involutus, Pi transporters, Pi-starvation, Pinus sylvestris, RNA-seq.
Year of publishing:2021
Number of pages:69-83
Numbering:31, 1
PID:20.500.12556/RUNG-7315 New window
COBISS.SI-ID:106352131 New window
DOI:10.1007/s00572-020-01001-6 New window
NUK URN:URN:SI:UNG:REP:SC4FDNZO
Publication date in RUNG:03.05.2022
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Record is a part of a journal

Title:Mycorrhiza
Year of publishing:2021

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License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:03.05.2022

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