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Title:Changes in exhaled volatile organic compounds following indirect bronchial challenge in suspected asthma
Authors:ID Peel, Adam (Author)
ID Wang, Ran (Author)
ID Ahmed, Waqar (Author)
ID White, Iain R. (Author)
ID Wilkinson, Maxim (Author)
ID Loke, Yoon K. (Author)
ID Wilson, Andrew M. (Author)
ID Fowler, Stephen J. (Author)
Work type:Unknown
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Background Inhaled mannitol provokes bronchoconstriction via mediators released during osmotic degranulation of inflammatory cells, and, hence represents a useful diagnostic test for asthma and model for acute attacks. We hypothesised that the mannitol challenge would trigger changes in exhaled volatile organic compounds (VOCs), generating both candidate biomarkers and novel insights into their origin. Methods Participants with a clinical diagnosis of asthma, or undergoing investigation for suspected asthma, were recruited. Inhaled mannitol challenges were performed, followed by a sham challenge after 2 weeks in participants with bronchial hyper-responsiveness (BHR). VOCs were collected before and after challenges and analysed using gas chromatography–mass spectrometry. Results Forty-six patients (mean (SD) age 52 (16) years) completed a mannitol challenge, of which 16 (35%) were positive, and 15 of these completed a sham challenge. Quantities of 16 of 51 identified VOCs changed following mannitol challenge (p<0.05), of which 11 contributed to a multivariate sparse partial least square discriminative analysis model, with a classification error rate of 13.8%. Five of these 16 VOCs also changed (p<0.05) in quantity following the sham challenge, along with four further VOCs. In patients with BHR to mannitol distinct postchallenge VOC signatures were observed compared with post-sham challenge. Conclusion Inhalation of mannitol was associated with changes in breath VOCs, and in people with BHR resulted in a distinct exhaled breath profile when compared with a sham challenge. These differentially expressed VOCs are likely associated with acute airway inflammation and/or bronchoconstriction and merit further investigation as potential biomarkers in asthma.
Keywords:asthma, exhaled volatile organic compounds, pulmonology, breath metabolomics
Publication date:01.01.2023
Year of publishing:2023
Number of pages:str. 966-973
Numbering:Vol. 78, issue 10
PID:20.500.12556/RUNG-8328-23e0f584-7283-f81d-4295-e80b6ccbddda New window
COBISS.SI-ID:160141827 New window
ISSN on article:0040-6376
DOI:10.1136/thorax-2022-219708 New window
Publication date in RUNG:31.07.2023
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Shortened title:Thorax
Publisher:British Medical Association
COBISS.SI-ID:5412615 New window