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Title:Enzyme-immobilized hierarchically porous covalent organic framework biocomposite for catalytic degradation of broad-range emerging pollutants in water
Authors:ID Elmerhi, Nada (Author)
ID Al-Maqdi, Khadega (Author)
ID Athamneh, Khawlah (Author)
ID Khayum Mohammed, Abdul (Author)
ID Škorjanc, Tina (Author)
ID Gándara, Felipe (Author)
ID Raya, Jesus (Author)
ID Simon, Pascal (Author)
ID Siri, Olivier (Author)
ID Trabolsi, Ali (Author)
Files:.pdf 1-s2.0-S0304389423015443-main.pdf (1,45 MB)
MD5: 0D204D36C1B0AABECE00B19E1004FDBF
Work type:Unknown
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Efficient enzyme immobilization is crucial for the successful commercialization of large-scale enzymatic water treatment. However, issues such as lack of high enzyme loading coupled with enzyme leaching present challenges for the widespread adoption of immobilized enzyme systems. The present study describes the development and bioremediation application of an enzyme biocomposite employing a cationic macrocycle-based covalent organic framework (COF) with hierarchical porosity for the immobilization of horseradish peroxidase (HRP). The intrinsic hierarchical porous features of the azacalix[4]arene-based COF (ACA-COF) allowed for a maximum HRP loading capacity of 0.76 mg/mg COF with low enzyme leaching (<5.0%). The biocomposite, HRP@ACA-COF, exhibited exceptional thermal stability (~200% higher relative activity than the free enzyme), and maintained ~60% enzyme activity after five cycles. LCMSMS analyses confirmed that the HRP@ACA-COF system was able to achieve >99% degradation of seven diverse types of emerging pollutants (2-mercaptobenzothiazole, paracetamol, caffeic acid, methylparaben, furosemide, sulfamethoxazole, and salicylic acid)in under an hour. The described enzyme-COF system offers promise for efficient wastewater bioremediation applications.
Keywords:covalent organic frameworks, enzymes, emerging pollutants, water purification, biocomposite
Publication date:01.10.2023
Year of publishing:2023
Number of pages:str. 1-9
Numbering:Vol. 459, [article no.] 132261
PID:20.500.12556/RUNG-8334-f9b2921a-3bbc-a88e-9e08-2f9ba22ba3a5 New window
COBISS.SI-ID:161219843 New window
ISSN on article:0304-3894
DOI:10.1016/j.jhazmat.2023.132261 New window
Publication date in RUNG:11.08.2023
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Record is a part of a journal

Title:Journal of hazardous materials
Shortened title:J. hazard. mater.
Publisher:Elsevier Scientific Publ. Co.
COBISS.SI-ID:25748224 New window


License:CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
Description:A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.