| Title: | Enzyme-immobilized hierarchically porous covalent organic framework biocomposite for catalytic degradation of broad-range emerging pollutants in water |
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| Authors: | ID Elmerhi, Nada (Author)
ID Al-Maqdi, Khadega (Author)
ID Athamneh, Khawlah (Author)
ID Khayum Mohammed, Abdul (Author)
ID Škorjanc, Tina (Author)
ID Gándara, Felipe (Author)
ID Raya, Jesus (Author)
ID Simon, Pascal (Author)
ID Siri, Olivier (Author)
ID Trabolsi, Ali (Author) |
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| Files: |  1-s2.0-S0304389423015443-main.pdf (1,45 MB)
MD5: 0D204D36C1B0AABECE00B19E1004FDBF
 https://www.sciencedirect.com/science/article/pii/S0304389423015443
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| Language: | English |
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| Work type: | Unknown |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | UNG - University of Nova Gorica
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| Abstract: | Efficient enzyme immobilization is crucial for the successful commercialization of large-scale enzymatic water treatment.
However, issues such as lack of high enzyme loading coupled with enzyme leaching present challenges for the widespread adoption
of immobilized enzyme systems. The present study describes the development and bioremediation application of an enzyme
biocomposite employing a cationic macrocycle-based covalent organic framework (COF) with hierarchical porosity for the
immobilization of horseradish peroxidase (HRP). The intrinsic hierarchical porous features of the azacalix[4]arene-based COF
(ACA-COF) allowed for a maximum HRP loading capacity of 0.76 mg/mg COF with low enzyme leaching (<5.0%). The
biocomposite, HRP@ACA-COF, exhibited exceptional thermal stability (~200% higher relative activity than the free enzyme),
and maintained ~60% enzyme activity after five cycles. LCMSMS analyses confirmed that the HRP@ACA-COF system was able
to achieve >99% degradation of seven diverse types of emerging pollutants (2-mercaptobenzothiazole, paracetamol, caffeic acid,
methylparaben, furosemide, sulfamethoxazole, and salicylic acid)in under an hour. The described enzyme-COF system offers
promise for efficient wastewater bioremediation applications. |
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| Keywords: | covalent organic frameworks, enzymes, emerging pollutants, water purification, biocomposite |
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| Publication date: | 01.10.2023 |
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| Year of publishing: | 2023 |
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| Number of pages: | str. 1-9 |
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| Numbering: | Vol. 459, [article no.] 132261 |
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| PID: | 20.500.12556/RUNG-8334-f9b2921a-3bbc-a88e-9e08-2f9ba22ba3a5  |
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| COBISS.SI-ID: | 161219843 |
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| UDC: | 54 |
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| ISSN on article: | 0304-3894 |
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| DOI: | 10.1016/j.jhazmat.2023.132261 |
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| NUK URN: | URN:SI:UNG:REP:VOLGXDE1 |
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| Publication date in RUNG: | 11.08.2023 |
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| Views: | 2185 |
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| Downloads: | 11 |
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