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Title:In vitro tumor hypoxia imaging with fluorescent covalent organic frameworks
Authors:ID Skorjanc, Tina (Author)
ID Shetty, Dinesh (Author)
ID Makuc, Damjan (Author)
ID Mali, Gregor (Author)
ID Bergant Marušič, Martina (Author)
ID Valant, Matjaž (Author)
Files:URL https://skd2023.chem-soc.si/wp-content/uploads/2023/08/SKD-2023-Programska-knjizica-110823-3.pdf
 
Language:Slovenian
Work type:Not categorized
Typology:1.12 - Published Scientific Conference Contribution Abstract
Organization:UNG - University of Nova Gorica
Abstract:Hypoxia refers to a condition where cells and tissues experience low, inadequate levels of O2. While healthy tissues are typically supplied with sufficient O2 (normoxia), cancerous tissues commonly face hypoxia due to the tumor’s extraordinarily high demand for oxygen. Various fluorescent small-molecule probes have been designed for selective detection of hypoxia in living cells, but few nanomaterials have been investigated for this type of bioimaging. Herein, we prepare a fluorescent covalent organic framework (COF) with β-ketoenamine linkages and post-synthetically modify it to conjugate hypoxia-sensitive nitroimidazole moieties into its pores (NI-COF). Stacks of sheets in NI-COF observed under electron microscopy were exfoliated by ultrasonication, and dynamic light scattering measurements confirmed particle size of less than 200 nm. Thus-prepared material exhibited good stability in physiological conditions and low cytotoxicity in in vitro experiments. NI-COF also showed useful fluorescence properties with an emission peak at 490 nm (λex = 420 nm) at both neutral and mildly acidic pH levels that are characteristic of tumor tissues. Encouraged by the favorable properties of the material, we incubated HeLa cells pre-treated in either hypoxic or normoxic conditions with NI-COF. Fluorescence microscopy images demonstrated that the material was preferentially taken up by hypoxic cells, which showed higher fluorescence signal in their interior than cells cultured under normoxia conditions. It is anticipated that this study will stimulate further developments of COFs for imaging various biological conditions.
Keywords:hypoxia, fluorescence, covalent organic frameworks, imaging, tumor cells
Year of publishing:2023
PID:20.500.12556/RUNG-8438 New window
COBISS.SI-ID:164896003 New window
NUK URN:URN:SI:UNG:REP:UNKHHIIL
Publication date in RUNG:19.09.2023
Views:943
Downloads:3
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Record is a part of a monograph

Title:Slovene Chemical Days 2023
Place of publishing:Portorož
Year of publishing:2023
Conference organizer:Slovensko kemijsko društvo

Document is financed by a project

Funder:EC - European Commission
Funding programme:H2020
Project number:101038091
Name:Novel COF-based sensors for detecting organic agents in water
Acronym:COFsensor

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