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Title:Quantitative detection of Microcystis aeruginosa (cyanobacteria) in water using single domain antibodies (VHH) : dissertation
Authors:ID Oginni, Gbenga Folorunsho (Author)
ID De Marco, Ario (Mentor) More about this mentor... New window
ID Dolinar, Marko (Author of conversation)
Files:.pdf FINAL_APPROVED_GBENGA_OGINNI_DISERTATION_14_5_2024.pdf (3,80 MB)
MD5: E7E04408BCE9E8528FF489B749EED1FD
 
Language:English
Work type:Doctoral dissertation
Typology:2.08 - Doctoral Dissertation
Organization:FPŠ - Graduate School
Abstract:Microcystis aeruginosa accumulation in freshwater poses a significant threat to aquatic organisms and human health. The toxicity of Cyanobacteria metabolites urges for the development of methods for their rapid and efficient detection but what is still almost completely missing is the availability of reagents for the quantification of M. aeruginosa cells in water to monitor the fluctuations of its population. In this study, nanobodies against cell surface antigens of the toxic Cyanobacteria M. aeruginosa were recovered bywhole-cell panning of a naive phage display library. Six unique sequences were identified and three of them sub-cloned and purified as fusion immunoreagents together with either green fluorescent protein or Avi-Tag to be used for diagnostics. Theirspecificity and sensitivity were evaluated by immunofluorescence, by fluorescent and colorimetric cell ELISA and by thermal lens spectrometry (TLS). No cross-reactivity with unrelated microalgae was detected, and both ELISA and TLS methods provided a linear range of detection of several logs. The limit-of-detection of TLS was as low as 1 cell/ml.
Keywords:cyanobacteria, nanobodies, phage display, thermal lens spectrometry, dissertations
Publication status:Published
Publication version:Version of Record
Place of publishing:Nova Gorica
Place of performance:Nova Gorica
Publisher:G. F. Oginni
Year of publishing:2024
Year of performance:2024
Number of pages:XXI, 118 str.
PID:20.500.12556/RUNG-9204 New window
COBISS.SI-ID:206926083 New window
UDC:577
NUK URN:URN:SI:UNG:REP:C731QQ3O
Publication date in RUNG:10.09.2024
Views:643
Downloads:11
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Secondary language

Language:Slovenian
Title:Disertacija
Keywords:cianobakterije, nanotelesa, fagni prikaz, spektrometrija termičnih leč, disertacije


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