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Title:Early stress detection in forest trees using a nanobody-functionalized electrochemical biosensor for ascorbate peroxidase
Authors:ID D'Ercole, Claudia (Author)
ID Svigelj, Rossella (Author)
ID Mrak, Tanja (Author)
ID De Marco, Ario (Author)
Files:.pdf published_article.pdf (4,65 MB)
MD5: 658E6511CA2E3E92CD5950EA9A7D4915
 
URL https://www.sciencedirect.com/science/article/pii/S2667064X25001095?via%3Dihub
 
Language:English
Work type:Unknown
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature such as heavy metal contamination, drought, or pest infestations which may lead to their massive decline. We designed a comprehensive approach for isolating, producing and functionalizing reagents suitable for the affordable detection of forest plant stress biomarkers with the aim to provide quantitative data to assess plant stress fluctuation and, possibly, to design mitigation strategies. We first optimized a panning protocol to recover nanobodies targeting shared sequences that could cross-react with both Pisum sativum and Populus nigra ascorbate peroxidase (APX). After their production as recombinant constructs and their extensive biophysical and biochemical characterization, such reagents were exploited as the immunocapture element of an electrochemical biosensor conceived as a potential point-of-care device. Such biosensor could detect both pea and poplar APX in leaf extracts and could be used to clearly discriminate between control and heavy metal-stressed poplar plants based on their APX activity, even before the appearance of any phenotypic symptom. The combination of fast and inexpensive reagent production with the development of portable diagnostics opens the opportunity for large-scale, on-site surveys of forest trees.
Keywords:plant stress, scavengers, diagnostics, nanobodies, biosensors
Publication status:Published
Publication version:Version of Record
Publication date:01.06.2025
Year of publishing:2025
Number of pages:8 str.
Numbering:Vol. 16, [article no.] ǂ100844
PID:20.500.12556/RUNG-9967 New window
COBISS.SI-ID:232353027 New window
UDC:577
ISSN on article:2667-064X
DOI:10.1016/j.stress.2025.100844 New window
NUK URN:URN:SI:UNG:REP:673K6SAJ
Publication date in RUNG:11.04.2025
Views:173
Downloads:2
Metadata:XML DC-XML DC-RDF
:
D’ERCOLE, Claudia, SVIGELJ, Rossella, MRAK, Tanja and DE MARCO, Ario, 2025, Early stress detection in forest trees using a nanobody-functionalized electrochemical biosensor for ascorbate peroxidase. Plant stress [online]. 2025. Vol. 16, no. 100844. [Accessed 16 April 2025]. DOI 10.1016/j.stress.2025.100844. Retrieved from: https://repozitorij.ung.si/IzpisGradiva.php?lang=eng&id=9967
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Record is a part of a journal

Title:Plant stress
Publisher:Elsevier B.V.
ISSN:2667-064X
COBISS.SI-ID:68373507 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Funding programme:Raziskovalni program
Project number:P4-0107
Name:Gozdna biologija, ekologija in tehnologija

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

Language:Undetermined
Abstract:Forest environments are exposed to multiple stressful factors of both abiotic and biotic nature such as heavy metal contamination, drought, or pest infestations which may lead to their massive decline. We designed a comprehensive approach for isolating, producing and functionalizing reagents suitable for the affordable detection of forest plant stress biomarkers with the aim to provide quantitative data to assess plant stress fluctuation and, possibly, to design mitigation strategies. We first optimized a panning protocol to recover nanobodies targeting shared sequences that could cross-react with both Pisum sativum and Populus nigra ascorbate peroxidase (APX). After their production as recombinant constructs and their extensive biophysical and biochemical characterization, such reagents were exploited as the immunocapture element of an electrochemical biosensor conceived as a potential point-of-care device. Such biosensor could detect both pea and poplar APX in leaf extracts and could be used to clearly discriminate between control and heavy metal-stressed poplar plants based on their APX activity, even before the appearance of any phenotypic symptom. The combination of fast and inexpensive reagent production with the development of portable diagnostics opens the opportunity for large-scale, on-site surveys of forest trees.


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