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1.
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
Jean-Christophe Rain, Laetitia Ligat, Ario de Marco, Emilie Lemesre, Laura Keller, Virginie Bernard, Nicolas Bery, Sandrine Moutel, Gilles Fevre, Aurelien Olichon, Franck Perez, 2016, original scientific article

Abstract: In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1). Conformation antibodies against active RHO GTPase were also obtained. Selected hs2dAb were used in various immunoassays and were often found to be functional intrabodies, enabling tracking or inhibition of endogenous targets. Functionalization of intrabodies allowed specific protein knockdown in living cells. Finally, direct selection against the surface of tumor cells produced hs2dAb directed against tumor-specific antigens further highlighting the potential use of this library for therapeutic applications.
Found in: ključnih besedah
Keywords: nanobodies, synthetic phage display library, in vitro panning
Published: 17.08.2016; Views: 3219; Downloads: 198
.pdf Fulltext (4,32 MB)

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Development of encapsulation techniques for the production and conservation of synthetic seeds in ornamental plants
Yelda Ozden-Tokatli, Anna De Carlo, Fusun Gumusel, Sara Pignattelli, Maurizio Lambardi, 2008, original scientific article

Abstract: The synthetic seed ("synseed") technology, initially developed through the encapsulation of somatic embryos, is now available for the use with non-embryogenic in vitro-derived explants, such as buds and nodal segments. For ornamental plants, to date there are few reports dealing with the production and the conservation of synseeds. Hence, the present study was conducted to develop an efficient encapsulation protocol for apical and axillary buds from various ornamental shrubs, i.e., oleander (Nerium oleander L.), photinia (Photinia fraseri Dress.), and lilac (Syringa vulgaris L.). For encapsulation, apical and axillary buds were excised, directly immersed in a Na-alginate solution, and then released dropwise in 100 mM CaCl2 center dot 2H(2)O for bead hardening. With photinia, best results were achieved when the synseeds were prepared using 3% Na-alginate, beads hardened for 30 min and germinated on hormone-free or BA-containing gelled MS medium. Thus, up to 92% of synseeds germinated and converted to shoots in a period of 10-11 days. The synthetic seeds of lilac, containing apical buds, showed a shorter germination time when 10-30 g 1(-1) sucrose was included in their "artificial endosperm". Successful medium-term conservation was then achieved with the storage of synthetic seeds at 4 degrees C in the dark on gelled MS medium, where up to 75% (oleander) and and 9 1 % (photinia) synseeds still germinated after 2 or 3 months of cold storage, respectively. Moreover, promising results have been obtained with the cryopreservation of photinia apical buds by means of the technique of "encapsulation-dehydration".
Found in: ključnih besedah
Keywords: conservation, cryopreservation, encapsulation, ornamentals, synthetic seeds
Published: 20.04.2020; Views: 1282; Downloads: 0
.pdf Fulltext (3,94 MB)

5.
Merohedral twinning in protein crystals revealed a new synthetic three helix bundle motif
Matteo De March, S. Geremia, 2012, complete scientific database or corpus

Found in: ključnih besedah
Keywords: crystal structures, de novo protein, synthetic construct
Published: 16.03.2021; Views: 553; Downloads: 2
URL Fulltext (0,00 KB)

6.
Structural basis of human clamp sliding on DNA
Matteo De March, N. Merino, S. Barrera-Vilarmau, R. Crehuet, S. Onesti, Francisco J. Blanco-Silva, A. De Biasio, 2018, complete scientific database or corpus

Found in: ključnih besedah
Summary of found: ...DNA binding protein, Homo sapiens, synthetic constructs, Escherichia coli...
Keywords: DNA binding protein, Homo sapiens, synthetic constructs, Escherichia coli
Published: 17.03.2021; Views: 708; Downloads: 2
URL Fulltext (0,00 KB)

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