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Title:Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma Patient Derived Xenografts
Authors:ID Crepin, Ronan, Institut Curie (Author)
ID Gentien, David, Institut Curie (Author)
ID Duche, Angeline, Institut Curie (Author)
ID Rapinat, Audrey, Institut Curie (Author)
ID Reyes, Cecile, Institut Curie (Author)
ID Nemati, Fariba, Institut Curie (Author)
ID Massonnet, Gerald, Institut Curie (Author)
ID Deacaudin, Didier, Institut Curie (Author)
ID Djander, Selma, Institut Curie (Author)
ID Moutel, Sandrine, Institut Curie (Author)
ID Desrumeaux, Klervi Even, Institut Curie (Author)
ID Cassoux, Nathalie, Institut Curie (Author)
ID Piperno-Neumann, Sophie, Institut Curie (Author)
ID Amigorena, Sebastian, Institut Curie (Author)
ID Perez, Franck, Institut Curie (Author)
ID Roman-Roman, Sergio, Institut Curie (Author)
ID De Marco, Ario, UNG (Author)
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Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:UNG - University of Nova Gorica
Abstract:Monoclonal antibodies specific for biomarkers expressed on the surface of uveal melanoma (UM) cells would simplify the immune-capture and genomic characterization of heterogeneous tumor cells originated from patient derived xenografts (PDXs). Antibodies against four independent tumor antigens were isolated by panning a nanobody synthetic library. Such antibodies enabled flow-cytometry-based sorting of distinct cell sub-populations from UM PDXs and to analyze their genomic features. The complexity and specificity of the biochemical and genomic biomarker combinations mirrored the UM tumor polyclonality. The data showed that MUC18 is highly and universally displayed at the surface of UM cells with different genetic background and consequently represents a reliable pan-biomarker for their identification and purification. In contrast, the other three biomarkers were detected in very variable combinations in UM PDX cells. The availability of the identified nanobodies will be instrumental in developing clone-specific drug evaluation and rational clinical strategies based on accurate genomic profiling
Keywords:nanobodies, uveal melanoma, patient derived xenografts, MUC18, membrane surface biomarkers, panning, tumor polyclonality
Publication version:Version of Record
Year of publishing:2017
Number of pages:11
PID:20.500.12556/RUNG-3064-55912669-33a0-6520-c8d7-18453b1b60b4 New window
COBISS.SI-ID:4768763 New window
DOI:10.1111/pcmr.12577 New window
Publication date in RUNG:19.04.2017
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Record is a part of a journal

Title:Pigment Cell and Melanoma Research
Publisher:Wiley: 12 months
Year of publishing:2017


License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:19.04.2017